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  • Leptospirosis and characterization of Leptospira isolates from patients in Koprivnica-Križevci County, Croatia from 2000–2004 [Elektronski vir]
    Mišić-Majerus, Ljiljana ; Cerar Kišek, Tjaša, 1980- ; Ružić-Sabljić, Eva
    Introduction. Leptospirosis, caused by spirochetes of the genus Leptospira, is present in the Koprivnica-Križevci County area, Croatia. Clinical manifestation can range from asymptomatic, short-term ... mild, non-specific febrile disease, to severe forms with high mortality rates. Aim. The aim of the study was to valuate culture in front of microscopic agglutination test (MAT) for diagnosis of infection, and to evaluate clinical and laboratory features of the disease. Moreover, we want to characterize Leptospira strains involved in infection in Koprivnica-Križevci County, Croatia. Methods. We included 68patients with clinical presentation consistent with leptospirosis collected in a 5-year period (2000–2004). Clinical samples (blood, urine and cerebrospinal fluid, CSF) were inoculated in Kolthoff’s medium; species of isolated Leptospira strains was determined with Tm of real-time PCR, serogroup/serovar with MAT and NotI-RFLP analysis. Demonstration of specific antibodies in patients’ sera was done using microscopic agglutination test. Results. Leptospira was isolated from the blood of 14/51(27.5%) patients and the most often identified serogroup/serovar was Icterohaemorrhagiae (8/10, 80%) followed by Grippotyphosa (10%). Regarding to species level, 8/10 isolated belonged to L. interrogans sensu stricto and one to L. kirschneri species. MAT was carried out on 51patients with suspected leptospirosis, and was positive in 11/51(21.5%) patients. Most of our patients presented with moderate severe symptoms, were hospitalized from August to October, and were infected mainly during the work or recreation in our county. The frequency of particular clinical features and pathological laboratory findings correlated with the severity of the clinical condition. Conclusions. Leptospirosis can be confirmed microbiologically, culture and MAT contributed almost equally to the diagnosis of infection. Serovar Icterohaemorrhagiae was found as the dominant one, and L. interrogans sensu stricto as dominant species in our county. Epidemiological data shown that leptospirosis occurs seasonally, affects the rural population, and most commonly is presented with moderate severe clinical course.
    Source: Access microbiology. - ISSN 2516-8290 (Vol. 5, iss. 4, 2023, str. 1-12)
    Type of material - e-article ; adult, serious
    Publish date - 2023
    Language - english
    COBISS.SI-ID - 168212995

    Link(s):

    https://www.microbiologyresearch.org/content/journal/acmi/10.1099/acmi.0.000431
    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10202403/pdf/acmi-5-431.pdf
    Repository of the University of Ljubljana – RUL
    DOI

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