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Localization of mRNAs encoding acetylcholinesterase and butyrylcholinesterase in the rat spinal cord by nonradioactive in situ hybdrizationMiš, Katarina ...In spite of intensive investigations, the roles of acetylcholinesterase (AChE;EC 3.1.1.7) and butyrylcholinesterase (BuChE; EC 3.1.1.8) in the centralnervous system (CNS) remain unclear. A role ... recently proposed for BuChEas an explanation for survival of AChE knockout mice is compensation for AChE activity if it becomes insufficient. Neuronal contribution of both enzymes to the cholinesterase pool in the neuromuscular junction has also beensuggested. These proposals imply that BuChE expression follows that of AChE and that, in addition to AChE, BuChE is also expressed in a-motor neurons. However, these assumptions have not yet been properly tested. Histochemical approaches to these problems have been hampered by a number of problems that prevent unambiguous interpretation of results. In situ hybridization (ISH) of mRNAs encoding AChE and BuChE, which is the state-of-the-art approach, has not yet been done. Here we describe rapid nonradioadive ISH for the localization of mRNAs encoding AChE and BuChE. Various probes and experimental conditions had been tested to obtain reliable localization. In combination with RT-PCR, ISH revealed that, in rat spinal cord, cells expressing AChE mRNA also express BuChE mRNA but in smaller quantities. a-Motor neurons had the highest levels of both mRNAs. Virtual absence of transcripts encoding AChE and BuChE in glia might reflect a discrepancy between mRNA and enzyme levels previously reported for cholinesterases.Source: The Journal of histochemistry and cytochemistry. - ISSN 0022-1554 (Letn. 51, št. 12, 2003, str. 1633-1644)Type of material - article, component partPublish date - 2003Language - englishCOBISS.SI-ID - 17221337
Author
Miš, Katarina |
Marš, Tomaž, 1972- |
Jevšek, Marko |
Brank, Martina |
Zajc-Kreft, Katarina |
Grubič, Zoran, 1948-
Topics
Spinal Cord |
Enzymology |
Rna, Messenger |
Acetylcholinesterase |
Metabolism |
Genetics |
Butyrylcholinesterase |
Metabolism |
Genetics |
Rats, Wistar |
Polymerase Chain Reaction |
Rna Probes |
In Situ Hybridization |
Immunohistochemistry |
Fluorescent Dyes |
Cell Count |
Acetilholinesteraza |
Butirilholinesteraza |
Hrbtenjača |
RNA, prenašalna |
Celica, štetje |
Fluorescentna barvila |
Imunohistokemija |
In situ hibridizacija |
Podgane, Wistar |
Polimerazna, verižna reakcija |
RNA sonde
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Database name | Field | Year |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
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Miš, Katarina | 19318 |
Marš, Tomaž, 1972- | 16345 |
Jevšek, Marko | 22403 |
Brank, Martina | 11903 |
Zajc-Kreft, Katarina | 13309 |
Grubič, Zoran, 1948- | 04410 |
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