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Uporaba različnih sevov bakterije Borrelia afzelii za dokaz protiteles IgM in IgG s testom indirektne imunofluoescence (IFT) = Application of different strains of bacterium Borrelia afzelii for detecting IgM and IgG antibodies with indirect immunofluorescence assay (IFA) : diplomska nalogaSekne, JanaLajmska borelioza je najbolj razsirjena zoonoza na severni polobli, ki jo povzroèa spiralna bakterija Borrelia burgdorferi sensu lato. B. burgdorferi sensu lato je kompleks najmanj treh za èloveka ... patogenih borelijskih vrstČ B. afzelii, B. garinii in B. burgdorferi sensu stricto. Povzroèiteljico bolezni prenasajo predvsem klopi iz rodu Ixodes. Gostitelji so razlièni sesalci, glodalci in ptice. Èlovek je le nakljuèni gostitelj. Do okuzbe pri èloveku pride na mestu vboda klopa, kjer nastane znaèilna kozna sprememba erythema migrans. V primeru neznaèilnih kliniènih znakov je potrebna mikrobioloska potrditev borelijske okuzbe. Vse pogosteje se uporabljajo seroloski testi, s katerimi ugotavljamo specifièna protitelesa v razliènih bioloskih materialih. Postavitev diagnoze je tezavna, saj klinièna slika moèno variira glede na geografsko razsirjenost borelijskih vrst, imunski odziv pa glede na antigenskovariabilnost borelij. Borelije namreè na svoji zunanji membrani izrazajo razliène zunanje povrsinske proteine, imenovane Osp, ki sprozijo imunski odziv. Namen nase naloge je bil, da dokazemo najprej specifièna protitelesa z razliènima antigenoma, nato pa primerjati rezultate med seboj inugotoviti, koliksne razlike v vrednostih protiteles IgM in IgG nastanejo pridoloèanju z enim ali drugim antigenom (B. afzelii AB ali B. afzelii ABC). Vkonèni fazi smo hoteli ugotoviti, kateri antigen bi bil primernejsi za dokaz borelijske okuzbe. Analizirali smo 120 serumov s sumom na lajmsko boreliozo, vkaterih smo hoteli dokazati specifièna protitelesa razredov IgM in IgG. Doloèali smo jih z dvema razliènima antigenoma B. afzelii in s testom indirektne imunofluorescence (IFT). Ugotovili smo, da z antigenom B. afzelii ABC doloèimo veè bolnikov s protitelesi IgM, z antigenom B. afzelii AB pa veè bolnikov s protitelesi IgG. Torej igra pomembno vlogo OspC antigen, ki specifièno sprozi nastanek protiteles IgM v zgodnji fazi bolezni. Z obema antigenoma smo doloèili popolno ujemanje rezultatov pri doloèanju protiteles IgM v 104ž120 (86,7 %) primerov, pri doloèanju protiteles IgG pa nekoliko manj, in sicer v 86ž120 (71,7 %) primerov. Ugotavljali smo tudi, pri kaksnem delezu preiskovancev smo ugotovili enak titer protiteles z dvema razliènima antigenomaČ pri doloèanju protiteles IgM v 87ž120 (72,5 %) primerih, pri doloèanju protiteles IgG nekoliko manj in sicer v 75ž120 (62,5 %) primerih. Ugotovimo lahko, da z obema antigenoma pri testiranju z IFT dobimo podobne rezultate in da sta oba antigena uporabna za diagnostièno delo v laboratoriju,vendar je antigen B. afzelii ABC bolj pomemben za dokaz protiteles IgM kakor IgG in obratno, antigen B. afzelii AB ima veèji pomen za dokaz protiteles IgG.Type of material - undergraduate thesisPublication and manufacture - Ljubljana : [Sekne, J.], 2005Language - slovenianCOBISS.SI-ID - 1758577
Author
Sekne, Jana
Other authors
Marin, Jožica |
Ružić-Sabljić, Eva
Topics
Borrelia burgdorferi |
serotipizacija borelij |
borelioza |
zdravljenje |
metode
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Library/institution |
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Faculty of Pharmacy, Ljubljana | Ljubljana | FFALJ |
reading room 1 cop.
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Database name | Field | Year |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
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Sekne, Jana | ![]() |
Marin, Jožica | 03172 |
Ružić-Sabljić, Eva | 13296 |
Source: Personal bibliographies
and: SICRIS
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