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  • Studying cell death initiation using a digital microscope [Elektronski vir]
    Arnšek, Tina ...
    Hypersensitive response (HR)-conferred resistance is an effective defense response that can be determined by the N resistance genes. HR is manifested as the formation of cell death zones on ... inoculated leaves. Here, a protocol for studying the rate of cell death initiation by imaging inoculated leaves in the time between the cell death initiation and the cell death appearance using a digital microscope is presented. The digital microscope enables a continuous imaging process in desired intervals, which allows an accurate determination of cell death initiation rate up to minutes exactly, as opposed to hours in traditional methods. Imaging with the digital microscope is also independent of light and can therefore be used during day and night without disturbing the circadian rhythm of the plant. Different pathosystems resulting in programmed cell death development could be studied using this protocol with minor modifications. Overall, the protocol thus allows simple, accurate, and inexpensive identification of cell death initiation rate.
    Source: Journal of visualized experiments [Elektronski vir]. - ISSN 1940-087X (Vol. 201, [article no.] 3027, 2023, str. 1-9)
    Type of material - e-article ; adult, serious
    Publish date - 2023
    Language - english
    COBISS.SI-ID - 177346819

    Link(s):

    https://www.jove.com/t/65824/studying-cell-death-initiation-using-a-digital-microscope
    DOI

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