-
Methimazole upregulates T-cell-derived cytokines without improving the existing Th1/Th2 imbalance in Grave's diseaseKocjan, Tomaž, 1966- ...There is probably a systemic shift of cytokine production in patients with Graves' disease (GD) toward the Th2 cytokine response. Methimazole (MMI) is the first choice for patients with GD and ... presumably has some direct immunomodulatory action. The aim of this study was to evaluate the balance shift in Th1/Th2 cytokines in patients with GD after 1 yr of MMI treatment, when compared to the same balance in patients with newly diagnosed GD before treatment and in healthy controls. Peripheral blood mononuclear cells (PBMC) were isolated from 17 healthy volunteers, from 18 patients with newly diagnosed GD before treatment and from 15 euthyroid patients with GD after 1 yr of MMI treatment. The PBMC were activated with ionomycin and phorbol 12-myristate 13-acetate (PMA). The concentrations of Th1/Th2 related cytokinesšinterferon (IFN)-gamma, interleukin (IL)-12 vs IL-4, IL-10đ in the culture supernatants were measured by ELISA. PBMC from patients with GD after treatment produced significantly more IFN-gamma and IL-4 than PBMC from patients with GD before treatment, but there were no significant differences in calculated ratios of Th1 against Th2 cytokines between these two groups. When compared to PBMC from healthy controls, PBMC from patients with GD after treatment produced significantly more IL-4 and significantly less IL-12. The calculated IL-12/IL-4 ratio after treatment was significantly lower than the same ratio from healthy controls. In conclusion, our results show no significant change in the ratio between Th1 and Th2 cytokines produced by PBMCfrom patients with GD after 1 yr of MMI treatment, when compared to the ratio before treatment. The ongoing prevalence of the Th2 immune response after treatment speaks against the immunomodulatory action of the drug on the systemic level.Source: Journal of endocrinological investigation. - ISSN 0391-4097 (Letn. 27, št. 4, 2004, str. 302-307)Type of material - article, component partPublish date - 2004Language - englishCOBISS.SI-ID - 17870553
Author
Kocjan, Tomaž, 1966- |
Wraber-Herzog, Branka |
Kocijančič, Andreja, 1942-2021 |
Hojker, Sergej
Topics
Graves' Disease |
Methimazole |
Monocytes |
Drug Effects |
Secretion |
Interferon Type II |
Interleukin-12 |
Ionomycin |
Tetradecanoylphorbol Acetate |
Interleukin-4 |
Interleukin-10 |
Enzyme-Linked Immunosorbent Assay |
Gravesova bolezen |
Inteferon tip II |
Interlevkin-12 |
Metimazol |
Monociti |
Encimsko-vezani imunosorbentni test |
Interlevkin-10 |
Interlevkin-4 |
Ionomicin |
Tetradekanoilforbol acetat
Shelf entry
Permalink
- URL:
Impact factor
Access to the JCR database is permitted only to users from Slovenia. Your current IP address is not on the list of IP addresses with access permission, and authentication with the relevant AAI accout is required.
Year | Impact factor | Edition | Category | Classification | ||||
---|---|---|---|---|---|---|---|---|
JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Select the library membership card:
DRS, in which the journal is indexed
Database name | Field | Year |
---|
Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
---|---|
Kocjan, Tomaž, 1966- | 15783 |
Wraber-Herzog, Branka | 04890 |
Kocijančič, Andreja, 1942-2021 | 03343 |
Hojker, Sergej | 01900 |
Select pickup location:
Material pickup by post
Notification
Subject headings in COBISS General List of Subject Headings
Select pickup location
Pickup location | Material status | Reservation |
---|
Please wait a moment.