ALL libraries (COBIB.SI union bibliographic/catalogue database)
-
Digital PCR for the characterization of reference materials [Elektronski vir]Cleveland, Megan H. ...Well-characterized reference materials support harmonization and accuracy when conducting nucleic acid-based tests (such as qPCR); digital PCR (dPCR) can measure the absolute concentration of a ... specific nucleic acid sequence in a background of non-target sequences, making it ideal for the characterization of nucleic acid-based reference materials. National Metrology Institutes are increasingly using dPCR to characterize and certify their reference materials, as it offers several advantages over indirect methods, such as UV-spectroscopy. While dPCR is gaining widespread adoption, it requires optimization and has certain limitations and considerations that users should be aware of when characterizing reference materials. This review highlights the technical considerations of dPCR, as well as its role when developing and characterizing nucleic acid-based reference materials.Source: Molecular aspects of medicine. - ISSN 1872-9452 (Vol. 96, [article no.] 101256, 2024, str. 1-8)Type of material - e-article ; adult, seriousPublish date - 2024Language - englishCOBISS.SI-ID - 186484995
Digital content: dCOBISS
| Author | Cleveland, Megan H. ... |
| Title | Digital PCR for the characterization of reference materials [Elektronski vir] |
| Publication date | 2024-02-14 |
| COBISS.SI-ID | 186484995 |
| Publication version in repository | Postprint, author's finally reviewed version, accepted for publication |
| Publication licence | Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International |
| Embargo | Postpone publication for public until 2025-02-14 |
Project(s) from which the publication was funded
| Title | Acronym | Project ID | Funder |
|---|---|---|---|
| Biotehnologija in sistemska biologija rastlin | P4-0165-2022 |
Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije |
Files that belong to the publication
https://www.sciencedirect.com/science/article/pii/S0098299724000153?via%3Dihub
Source: Personal bibliographies
and: SICRIS
