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Razvoj bioanalizne metode za določanje plazemskih koncentracij protimikrobnih učinkovin s tekočinsko kromatografijo visoke ločljivosti = Development of bioanalytical method for determining plasma concentrations of antimicrobial agents using high performance liquid chromatography : magistrska naloga : magistrski študijski program Industrijska farmacijaBenda, KlavdijaProtibakterijske učinkovine (β-laktami, glikopeptidi, aminoglikozidi, makrolidi, kinoloni in tetraciklini) sodijo med protimikrobne učinkovine, namenjene zdravljenju okužb. Kljub njihovi varnosti je ... za optimizacijo zdravljenja pri bolnikih s sepso smiselno uporabiti terapevtsko spremljanje koncentracije, saj se farmakokinetika protimikrobnih zdravil spreminja. Poleg tega se lahko za zmanjšanje vnetnega odziva pri bolnikih s sepso uporabljajo tudi hemoadsorbenti, ki lahko vežejo zdravila. V okviru magistrske naloge smo razvili bioanalizno metodo za sočasno določanje plazemskih koncentracij imipenema, amoksicilina, cefepima, meropenema, vankomicina in piperacilina v goveji plazmi. Plazemske vzorce smo pripravljali z obarjanjem proteinov z acetonitrilom in ekstrakcijo v diklorometanu. Vzorce smo analizirali s tekočinsko kromatografijo visoke ločljivosti, kjer smo za separacijo analitov uporabili C18 stacionarno fazo in mobilno fazo, ki je vsebovala 0,5 % H3PO4 (pH 7,0) in 0,5 % H3PO4 (pH 3,0) z dodatkom acetonitrila v razmerju 3 : 1 (v/v). Pretok mobilne faze je bil 1,3 mL/min. Analite smo detektirali z UV/VIS detektorjem. Metodo smo uspešno validirali v skladu s smernicami Evropske agencije za zdravila za validacijo bioanalizne metode. Na podlagi validacijskih parametrov (znotraj dnevna in med dnevna ponovljivost, točnost, specifičnost) lahko potrdimo, da je metoda primerna za analiziranje izbranih analitov v govejih plazemskih vzorcih. Območje linearnosti za cefepim, piperacilin, meropenem smo definirali v koncentracijskem območju 1–75 µg/mL, medtem ko je za vankomicin in amoksicilin 1–100 µg/mL, za imipenem pa 2,5–100 µg/mL. Vzorci za analizo so stabilni vsaj 12 ur pri 5 °C v avtomatskem vzorčevalniku. Validirano metodo smo uspešno uporabili za določanje koncentracij izbranih antibiotikov v in vitro poskusu vezave antibiotika na hemoadsorbent Cytosorb®. Ugotovili smo, da dodatek Cytosorba® vpliva na koncentracijo izbranih antibiotikov v goveji plazmi, kar kaže na vezavo antibiotikov na hemoadsorbent.Type of material - master's thesis ; adult, seriousPublication and manufacture - Ljubljana : [K. Benda], 2024Language - slovenianCOBISS.SI-ID - 192471811
Author
Benda, Klavdija
Other authors
Vovk, Tomaž, 1972-
Topics
bioanalizna metoda |
validacija |
protibakterijske učinkovine |
optimizacija priprave vzorcev
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Library/institution |
City | Acronym | For loan | Other holdings |
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Faculty of Pharmacy, Ljubljana | Ljubljana | FFALJ |
reading room 1 cop.
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Database name | Field | Year |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
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Benda, Klavdija | ![]() |
Vovk, Tomaž, 1972- | 18155 |
Source: Personal bibliographies
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