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  • Inhibition of Salmonella-induced IL-8 synthesis and expression of Hsp70 in enterocyte-like Caco-2 cells after exposure to non-starter lactobacilli
    Nemeth, Edina ...
    Oral administration of lactobacilli as probiotics is gaining importance in thetreatment of intestinal inflammations. We investigated the effect of non-starter lactobacilli Lactobacillus casei subsp ... casei 2756, Lactobacillus curvatus 2775, and Lactobacillus plantarum m2142 as well as their spent culture supernants (SCS) on Salmonella enteritidis 857 growth, interleukin (IL)-8 and heat shock protein 70 (Hsp 70) synthesis in undifferentiated crypt-like and differentiated villus-like Caco-2 cells. The cells were infected with graded numbers of non-starter lactobacilli or S. enteritidis 857for 1 h and allowed to recover for 24 h or exposed to 200 bacteria/cell for1 h and allowed to recover for different periods of time. In another experiment S. enteritidis 857 was first pre-treated with SCS-lactobacilli for 1 h before infevting the cells. The levels of IL-8 and Hsp70 were assessed using sandwich ELISA and immunostaining of Western blots, respectively. The effect of SCS-lactobacilli on S. enteritides 857 growth was evaluated by agar plate diffusion test. The non-starter lactobacilli induced a significant increase in the levels of both L-8 and Hsp7). However, compared with the S. eneritidis 857 induced IL-8 synthesis, the levels of IL-8 induced at the lactobacilli at any equivalent bacterial number were far lower. After exposure of Caco-2 cells to S. enteritidis 857 pre-treated with SCS-lactobacilli, it appeared that their SCS inhibited the S. enteritidis 857 growth and IL-8 synthesis and in addition induced the expression of Hsp70. The differences in response of crypt-and villus-like Caco-2 cells are merely areflection of their differentiation status. Our data suggest that the beneficial effect of non-starter lactobacilli to the intestinal inflammations might be associated with a decrease of the Il-8 levels. This effect could be mediated, at least in part, by the bacteria themselves or via a secreted antimicrobial product(s) either directly against the pathogens or indirectly through the synthesis of Hsp70.
    Source: International journal of food microbiology. - ISSN 0168-1605 (Letn. 112, št. 3, 2006, str. 266-274)
    Type of material - article, component part
    Publish date - 2006
    Language - english
    COBISS.SI-ID - 1947016

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