-
Gene electrotransfer of canine interleukin 12 into canine melanoma cell linesLampreht Tratar, Urša ...A gene electrotransfer (GET) of interleukin 12 (IL-12) had already given good results when treating tumors in human and veterinary clinical trials. So far, plasmids used in veterinary clinical ... studies encoded a human or a feline IL-12 and an ampicillin resistance gene, which is not recommended by the regulatory agencies to be used in clinical trials. Therefore, the aim of the current study was to construct the plasmid encoding a canine IL-12 with kanamycin antibiotic resistance gene that could be used in veterinary clinical oncology. The validation of the newly constructed plasmid was carried out on canine malignant melanoma cells, which have not been used in GET studies so far, and on human malignant melanoma cells. Canine and human malignant melanoma cell lines were transfected with plasmid encoding enhanced green fluorescence protein at different pulse parameter conditions to determine the transfection efficiency and cell survival. The IL-12 expression of the most suitable conditions for GET of the plasmid encoding canine IL-12 was determined at mRNA level by the qRT-PCR and at protein level with the ELISpot assay. The obtained results showed that the newly constructed plasmid encoding canine IL-12 had similar or even higher expression capacity than the plasmid encoding human IL-12. Therefore, it represents a promising therapeutic plasmid for further IL-12 gene therapy in clinical studies for spontaneous canine tumors. Additionally, it also meets the main regulatory agencies% (FDA and EMA) criteria.Source: The journal of membrane biology. - ISSN 0022-2631 (Vol. 248, iss. 5, 2015, str. 909-917)Type of material - article, component partPublish date - 2015Language - englishCOBISS.SI-ID - 1989499
Author
Lampreht Tratar, Urša |
Kamenšek, Urška |
Savarin, Monika |
Serša, Gregor |
Tozon, Nataša |
Omerzel, Maša, mag. farm. |
Brožič, Andreja |
Oliveira, Geraldo Gileno de Sá |
Nakagawa, Takayuki |
Saeki, Kohei |
Čemažar, Maja
Topics
interlevkin-12 |
melanom |
psi |
elektroporacija |
elektrogenska terapija |
canine IL-12 |
canine melanoma cell lines |
electroporation |
gene electrotransfer
Shelf entry
Permalink
- URL:
Impact factor
Access to the JCR database is permitted only to users from Slovenia. Your current IP address is not on the list of IP addresses with access permission, and authentication with the relevant AAI accout is required.
Year | Impact factor | Edition | Category | Classification | ||||
---|---|---|---|---|---|---|---|---|
JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Select the library membership card:
DRS, in which the journal is indexed
Database name | Field | Year |
---|
Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
---|---|
Lampreht Tratar, Urša | 36367 |
Kamenšek, Urška | 28387 |
Savarin, Monika | 35355 |
Serša, Gregor | 08800 |
Tozon, Nataša | 13328 |
Omerzel, Maša, mag. farm. | 34373 |
Brožič, Andreja | 35354 |
Oliveira, Geraldo Gileno de Sá | |
Nakagawa, Takayuki | |
Saeki, Kohei | |
Čemažar, Maja | 14575 |
Select pickup location:
Material pickup by post
Notification
Subject headings in COBISS General List of Subject Headings
Select pickup location
Pickup location | Material status | Reservation |
---|
Please wait a moment.