The ultrastructural colony architecture of six Clostridium difficile strains belonging to four different ribotypes (001, 027, 078/126 and 036) was studied by scanning electron microscopy (SEM). In ...2-day-old colonies several microareas differing in cell length and organization could be differentiated. Some cells at colony edges were extremely long and exhibited invasiveness into the agar. The ultrastructure of 5-day-old colonies is more homogenous and characterized by presence of sporulating cells, spores and extracellular matrix.
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