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  • Development and validation of a liquid chromatography tandem mass spectrometry assay for determination of raloxifene and its metabolites in human plasma
    Trontelj, Jurij ...
    This paper describes the development and validation of a method for the detection of raloxifene (Ral) and its two glucuronide metabolites, raloxifene-6-glucuronide (M1) and raloxifene-4_-glucuronide ... (M2), in human plasma samples. Both glucuronides were synthesized enzymatically, purified and used as authentic standards. The assay involves a simple solid phase extraction (SPE) procedure of 0.5mL of human plasma and subsequent analysis byLC-MS-MS. The recoveries were higher than 71% and chromatographic separationof all the analytes was accomplished in less than 7 min. Linear ranges (r2 > 0.99) were found from 0.200 to 340 _gžL, from 1.600 to 2720 _gžL and from 0.088 to 60.00 _gžL, for M1, M2 and Ral, respectively. The limits of detection achieved were 8, 11 and 6 ngžL for M1, M2 and Ral, respectively. Themethod presented was successfully applied to a genetic polymorphism study of 47 plasma samples from women taking Evista (raloxifene hydrochloride).
    Type of material - article, component part
    Publish date - 2007
    Language - english
    COBISS.SI-ID - 2107505