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Hipometilacija alfoidne DNA in klasične satelitne DNA na kromosomih 1, 9, 16 in Y v celicah ekstraembrionalnih tkiv : magistrska nalogaZagradišnik, BorisIntroduction. Regulation of DNA methylation is an elementary process in human genome. The pattern of 5-methylcytosines in normal somatic cells is tissue-specific and meticulously restored after each ... DNA replication. Both level and pattern of DNA methylation are set during early embryogenesis. Afterinitial global demethylation there is a surge in Ćde novoĆ DNA methylation. The final level of DNA methylation is lower in extraembryonic cells and in gamete-progenitor cells than in somatic embryonic tissue. Therefore, analysis of DNA methylation with fluorescein labeled antibody against 5-methylcytosine on metaphase chromosomes shows lower intensities of immunofluorescence in extraembryonic tissue than in normal human lymphocytes. Extraembryonic cells have lower overall immunofluorescence and lower immunofluorescence from centromeric and pericentromeric regions on chromosomes1, 9, 16 and from heterochromatin on chromosome Y in comparison with lymphocytes which exhibit very bright immunofluorescence. The main components of centromeric and pericentromeric regions of chromosomes are different types of non-coding repetitive DNA (i.e. classical satellite DNA). Hypomethylation of these repetitive sequences can be proposed to explain lowerimmunofluorescence from heterochromatin of metaphase chromosomes from human extraembryonic tissue. The aim of this study was to detect hipomethylation of alphoid DNA and classical satellite DNA on chromosomes 1, 9, 16 and Y in extraembyonic tissue in comparison with normal human leucocytesusing Southern blot method. Also results from immunofluorescence analysis of DNA methylation with antibody against 5 methylcytosine were evaluated. Methods. DNA methylation analysis was performed on DNA from chorionic villi, from placental fibroblasts and from normal adult human leucocytes. Methylation sensitive restriction enzymes were used for Southern blot analysis of DNA methylation. Results. (Abstract truncated at 2000 characters.)Type of material - master's thesisPublication and manufacture - Ljubljana : [B. Zagradišnik], 2000Language - slovenianCOBISS.SI-ID - 2120724
Author
Zagradišnik, Boris
Other authors
Kokalj-Vokač, Nadja
Topics
DNA methylation |
Genetics |
DNA methylation |
Physiology |
Chromosomes |
Metabolism |
DNA metilacija |
Genetika |
DNA metilacija |
Fiziologija |
Kromosomi |
Metabolizem |
biokemija |
genetika, biokemična
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Library/institution |
City | Acronym | For loan | Other holdings |
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MF, Central Medical Library, Ljubljana | Ljubljana | CMK |
outside loan 1 cop.
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Year | Impact factor | Edition | Category | Classification | ||||
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JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
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DRS, in which the journal is indexed
Database name | Field | Year |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
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Zagradišnik, Boris | 18205 |
Kokalj-Vokač, Nadja | 13343 |
Source: Personal bibliographies
and: SICRIS
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