Specificities of olfactory receptor neuron responses to amino acids in the black bullhead catfish (Ameiurus melas)
Dolenšek, Jurij ; Valentinčič, Tine
In vivo investigations of catfish olfactory receptor neurons (ORNs) were previously limited to studying responses of spontaneously active cells. The olfactory organ, however, also contains ORNs that ...lack spontaneous activity and respond to amino acids. To record electrical activity of ORNs that were inactive prior to stimulation, we bathed the olfactory organ with low conductive, highly purified water that reduces shunting and enables detection of action potentials from ORNs distant to the electrode. After stimulation with amino acids, these ORNs elicited either phasic-tonic or tonic only activities. The spike frequency of the phasic activity consisted of transient frequencies up to 108 Hz that lasted <450 ms. All tonic activities saturated at action potential frequencies of 17-21 Hz. Their durations were dose dependent over several log units of concentration as they closely followed that of the suprathreshold amino acid stimulation. Specificities of 44 ORNs were investigated with ten different amino acids tested at 10-4?M. Thirteen ORNs were excited by only one amino acid, l-norvaline, and 22 additional ORNs were excited by l-norvaline and l-methionine. Nine ORNs were excited by >2 amino acids that included l-norvaline. In 29 of 31 neurons responding to >1 amino acid, the duration of the responses to the most stimulatory amino acid was at least double compared to that to the other amino acids. The amplitude of electro-olfactogram (EOG) correlated significantly with the number of ORNs activated by the same amino acids confirming that the EOG represents the sum of ORN receptor potentials.
It was not necessary to add the material to the shelf.
Bibliographic material was successfully added on shelf.
Adding bibliographical material on shelf was not successful.
Material is already on the shelf.
Permalink
URL:
Impact factor
Access to the JCR database is permitted only to users from Slovenia. Your current IP address is not on the list of IP addresses with access permission, and authentication with the relevant AAI accout is required.
Year
Impact factor
Edition
Category
Classification
JCR
SNIP
JCR
SNIP
JCR
SNIP
JCR
SNIP
Select the library membership card:
DRS,
in which the journal is indexed
Database name
Field
Year
Links to authors' personal bibliographies
Links to information on researchers in the SICRIS system
Subject headings in COBISS General List of Subject Headings
Select pickup location
The material from the parent unit is free. If the material is delivered to the pickup location from another unit, the library may charge you for this service.
PDF
