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Optimizacija medija za proizvodnjo fitaz pri izbranih nitastih glivah : magistrsko delo = Media optimization for phytase production from selected filamentous fungi : master of science thesisRijavec Bregar, AndrejaNamen naloge je bil pridobiti biomaso različnih sevov glive Aspergillus ficuum s povečano fitazno aktivnostjo. Najboljše producente fitaz smo določili s kvalitativnim določanjem fitazne aktivnosti na ... PSM agarnih ploščah. S kultivacijo izbranih sevov na obogatenem mediju z virom dušika in fosforja smo ugotovili, da na fitazno aktivnost najbolje vpliva dodatek 5 mg NH4NO3/100 g substrata in 10 mg KH2PO4/100 g substrata. S kultivacijo šestih različnih sevov glive Aspergillus ficuum na optimiziranem mediju, pridobljena fitazno aktivna biomasa, poleg fitaz vsebuje tudi vrsto ostalih pomembnih encimov: celulaz, ksilanaz in amilolitičnih encimov. Največjo fitazno aktivnost smo določili sevu Aspergillus ficuum ZIM F031, sledita pa seva Aspergillus ficuum ZIM F040 in Aspergillus ficuum ZIM F032. Med 96 urno kultivacijo gliv smo spremljali produkcijo nastale biomase in razgradnjo fitinske kisline. Razgradnja fitinske kisline narašča s fitazno aktivnostjo in po 96 urah kultivacije posameznih sevov je njena vsebnost v substratu še med 35,6-52,6 % glede na začetek kultivacije. Zaradi ugodnih encimskih karakteristik je producirana biomasa primerna kot direktni krmni dodatek.Type of material - master's thesis ; adult, seriousPublication and manufacture - Ljubljana : [BF, Oddelek za živilstvo] : [A. Rijavec Bregar], 1999Language - slovenianCOBISS.SI-ID - 2169208
Author
Rijavec Bregar, Andreja
Other authors
Raspor, Peter |
Abram, Veronika
Topics
filamentozne glive |
Aspergillus ficuum |
fitaze |
fitinska kislina |
kultivacija gliv |
biomasa |
obogatitev substrata |
amilolitični encimi |
celulaze |
ksilanaze |
filamentous fungi |
Aspergillus ficuum |
phytase |
phytic acid |
cultivation of fungi |
biomass |
substrate supplementation |
amylolytic enzymes |
celullases |
xylanases
Library/institution |
City | Acronym | For loan | Other holdings |
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BF, Department of Food Science and Technology, Lj. | Ljubljana | BFZIV |
outside loan 2 cop.
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BF, Central Biotechnical Library, Ljubljana | Ljubljana | BFCBK |
outside loan 1 cop.
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Database name | Field | Year |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
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Rijavec Bregar, Andreja | 15945 |
Raspor, Peter | 04001 |
Abram, Veronika | 01522 |
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