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ON- and OFF-response of the photopic electroretinogram in relation to stimulus characteristics
Šuštar Habjan, Maja, 1979- ; Hawlina, Marko ; Brecelj, Jelka
The aim of this study was to explore the effect of stimulus duration, stimulusintensity, stimulus wavelength and background luminance on the amplitude and waveform of the ON- (b-wave) and OFF- ...(d-wave) response of the photopic ERG. The following parameters were used in this study: stimulus duration from 5 to 200 ms, the flash intensities from 0.4 to 2.1 log cd s/m(2), the background luminance from 20 to 50 cd/m(2) and stimulus wavelengths 460, 508 and 667 nm. Prolonging the stimulus duration from 75 to 200 ms did not influence the amplitudes of a-, b- and d-waves significantly. Higher stimulus intensities (1.9 log cd s/m(2)) broadened the b-wave and increased variability of its implicit time. With a brighter background (50 cd/m(2)) the b-wave did not broaden and its optimal amplitude was preserved. Stimuli of longer wavelengths (667 nm) significantly reduced the d-wave amplitude. Our results suggest that high intensities and longer wavelengths (red) may not be suitable stimulus parameters in the routine clinical ON- and OFF-response recording.
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