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Comparison of capillary electrophoresis and high performance liquid chromatography for determination of flavonoids in Achillea millefoliumKočevar Glavač, Nina ...Flavonoids represent an important bioactive component in Achillea millefolium.The comparison of the most commonly used analytical methods for the identification and quantification of flavonoids, ... capillary electrophoresis(CE) and high performance liquid chromatography (HPLC), is presented. The methods were optimized and validated. Using a 20 mM borate buffer with 30% (vžv) of methanol (pH 9.3) in the CE analysis and a gradient elution with water-acetonitrile mobile phase in the HPLC analysis, sufficient separation of the analytes was achieved. A relatively high injection volume inthe CE analysis (30 mbar * 30 s) enabled low limit of detection (0.3 to 0.7 mgžL). Repeatability of both methods was acceptable (relative standard deviation of peak area were < 6%). Additionally, the amount of flavonoids in areal sample of the dried herbal drug was determined.Source: Journal of pharmaceutical and biomedical analysis. - ISSN 0731-7085 (Vol. 46, 2008, str. 609-614)Type of material - article, component partPublish date - 2008Language - englishCOBISS.SI-ID - 2224753
Author
Kočevar Glavač, Nina |
Glavač, Igor |
Injac, Rade |
Kreft, Samo
Topics
Achillea millefolium |
kapilarna elektroforeza |
HPLC |
flavonoidi
source: Journal of pharmaceutical and biomedical analysis. - ISSN 0731-7085 (Vol. 46, 2008, str. 609-614)
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Database name | Field | Year |
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Kočevar Glavač, Nina | 26495 |
Glavač, Igor | |
Injac, Rade | 30616 |
Kreft, Samo | 15460 |
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