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Karakterizacija kazeinov iz kobiljega mleka = Characterization of caseins from mare's milkHobor, Sebastijan ...Kazeini predstavljajo najpomebnejšo beljakovinsko frakcijo v mleku zaradi njihovega pomena v prehrani novorojenca in gospodarskega pomena pri predelavi mleka. Delimo jih na Ca2+ občutljive (alfas1-, ... alfa s2-, ß-kazein) in na Ca2+ neobčutljive (K-kazein). Specifično vključevanje ključnih aminokislin in posttranslacijske modifikacije jim omogočajo edinstvene fuunkcionalne lastnosti (odpornost na visoke temperature, nimajo rigidne sekundarne/terciarne strukture, organiziranost kazeinov v micele). V četrtem eksonu gena CSN3 pri konju smo našli mutacijo (SNP) in posledično potencialno izgubo enega fosforilacijskega mesta na polipeptidni verigi, kar lahko vpliva na spremembo kemijskih/funkcionalnih lastnosti zrelega proteina. V ta namen smo želeli vzpostaviti sistem separacije posameznih kazeinskih frakcij, ki bi lahko predstavljal osnovo za standardizirano identifikacijo kazeinov iz kobiljega mleka. S SDS-PAGE je bila separacija kazeinskih proteinov pregroba. Z 2D-PAGE smo iz proteomskega profila kravjih laktoproteinov identificirali posamezne kazeine. S primerjavo kobiljih in kravjih laktoproteinov na 2-D gelu smo ugotovili, veliko večjo heterogenost kobiljih proteinov in zaradi tega ni bilo možno identificirati posameznih kazeinov kobile. V ta namen načrtujemo identifikacijo kobiljih kazeinov s pomočjo MS. Ta študija pomeni tudi prvi korak k nomenklaturi kazeinskih genov in njihovih alelnih variant pri konju. Študije kobiljega mleka postanejo vedno pomembnejše zaradi njihove visoke podobnosti s človeškimi laktoproteini in s tem realne možnosti uporabe kobiljega mleka za mlečne nadomestke pri dojenčkih, ki so alergični na laktoproteine v mleku prežvekovalcev.Source: Proteomika (Str. 61-69)Type of material - conference contributionPublish date - 2008Language - slovenianCOBISS.SI-ID - 2241672
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Database name | Field | Year |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
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Hobor, Sebastijan | 25515 |
Šmerc, Andreja | 29490 |
Jamnik, Polona, biotehnologinja | 18511 |
Kunej, Tanja, biologinja | 16361 |
Dovč, Peter, 1958- | 05098 |
Source: Personal bibliographies
and: SICRIS
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