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Fenotipska in genotipska karakterizacija bakterijskih izolatov vrste Bacillus cereus in sorodnih vrst : diplomska naloga = Phenotypic and genotypic characterization of bacterial isolates of Bacillus cereus and related species : graduation thesis
Bogataj, Andreja, 1979-
Aerobni sporogeni mikroorganizmi rodu Bacillus, še posebej vrste B. cereus, so pomembni v živilstvu kot povzročitelji kvara in kot potencialno zdravju škodljivi mikroorganizmi zaradi sinteze ...številnih encimov in toksinov. V delu je predstavljena primerjalna analiza izbranih fenotipskih in genotipskih značilnosti triinšestdesetih izolatov rodu Bacillus, med njimi desetih referenčnih sevov in triinpetdesetih izolatov iz različnih živil. Bakterije "skupine B. cereus" so kratke, relativno široke paličaste bakterije, sporangiji niso nabrekli. Ne asimilirajo manitola, so pa lecitinazno in hemolitično aktivne. Druge vrste rodu Bacillus imajo lahko posamične izmed omenjenih fizioloških lastnosti. Molekularna identifikacija izolatov z analizo regije 16S rDNK - ITS - 23S rDNK je uporabna za razlikovanje izolatov "skupine B. cereus" od ostalih vrst rodu Bacillus, ne pa tudi za razlikovanje izolatov znotraj "skupine B. cereus". Molekulska identifikacija bakterij z restrikcijsko analizo pomnoženega fragmenta gena cerAB, ki kodira cereolizin (fosfolipazo C in sfingomielinazo) omogoča identifikacijo in hitro razlikovanje zelo sorodnih sevov "skupine B. cereus", kar ni možno doseči z nobeno od ostalih metod, uporabljenih v tem delu.
Type of material - undergraduate thesis
; adult, serious
Publication and manufacture - Ljubljana : [BF, Oddelek za živilstvo] : [A. Bogataj], 1999
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