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  • APOBEC3 proteins inhibit LINE-1 retrotransposition in the absence of ORF1p binding
    Lovšin, Nika ; Peterlin, Matija Boris
    Members of the apolipoprotein B mRNA editing complex polypeptide 1-like (APOBEC) family of enzymes exhibit inhibitory activity against a variety of exogenous and endogenous retroviruses including ... retrotransposons, such as long interspersed element 1 (LINE-1). Indeed, human APOBEC3A, APOBEC3B, and APOBEC3F inhibit retrotrans-position of human LINE-1, mouse IAP and MusD retrotransposons. In our study, we examined whether the inhibitory effect of APOBEC3 proteins correlates with APOBEC3 ability to bind the LINE-1 ORF1 protein. We examined the interactions between the LINE-1 ORF1 protein and the most potent LINE-1 retrotransposon inhibitors, human APOBEC3A and APOBEC3B, by immunofluorescence and immunoprecipitation. Although human APOBEC3A shows the highest inhibitory potency against LINE-1 retrotransposon, no direct interactions were identified either by immunofluorescence or by co-immunoprecipitation. APOBEC3B binds to LINE-1 ORF1 protein, yet no co-localization was detected. We concluded that APOBEC3 proteins interfere indirectly with the LINE-1 retrotransposition pathway, probably through interference with RNA targeting.
    Source: Natural genetic engineering and natural genome editing : proceedings of the GenConText Research Symposium, 3-6 July 2008, Salzburg, Austria (Vol. 1178, no. 1, 2009, str. 268-275)
    Type of material - conference contribution
    Publish date - 2009
    Language - english
    COBISS.SI-ID - 23252263
    DOI

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