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  • Supplementation of rabbit diet with chestnut wood extract : effect on in vitro gas production from two sources of protein
    Kermauner, Ajda ; Lavrenčič, Andrej, 1965-
    In vitro gas production kinetics of soybean meal (SMB) and sunflower meal (SFM) were determined using three different inocula prepared from caecum contents of 78-day old rabbits of Slovenian meat ... line SIKA. The first inoculum was prepared from caecum contents of rabbit fed diet supplemented with 0.5% of shestnut wood extract (CWE) in the form of powder (POWD), the second one from caecum contents of rabbits fed diet supplemented with 0.6% of CWE coated with plant oil (COAT), while the third inoculum was prepared from caecum contents of rabbits not supplemented with CWE (CONT). Gas productions were measured over 60 h of incubation and obtained gas volumes were modelled with Gompertz model. The total potential gas productions (parameter "B" of the Gompertz model) were higher (P<0.05) when SMB and SFM were incubated in POWD (147 and 108 ml/g DM for SBM and SFM) than CONT (137 and 97 ml/g DM for SBM and SFM). The highest parameter B was obtained for SBM when COAT was used (170 ml/g DM), while for SFM the highest parameter B was obtained when POWD (108 ml/g DM) wasused. When both substrates were incubated in POWD they had shorter time of maximum fermentation rate (TMFR: 10.0 and 7.4 h for SBM and SFM) and higher maximum fermentation rate (MFR: 4.73 and 6.06 ml/h for SBM and SFM) than when incubated in CONT (TMFRs of 16.0 and 12.0 h for SBM and SFM, respectively, and MFRs of 3.58 and 4.07 ml/h for SBM and SFM, respectively) or COAT (TFMRs of 18.4 and 12.2 h and MFRs of 2.96 and 4.39 ml/h for SBM and SFM, respectively). In the first 8 h of fermentation, higher (P<0.05) amounts of gas were produced from substrates incubated in POWD (44 ml both SBM and SFM) than in CONT (24 and 20 ml for SBM and SFM, respectively) and COAT )34 and 27 ml for SBM and SFM, respectively). These results suggest that CWE fed as the powder reaches the caecum increasing the caecal microbial fermentation, while CWE coated with plant oils (COAT) decreases the activity of CWE in the caecum, especially in the first 8 hours of fermentation. The caecal microorganisms are assumed to degrade the coating around CWE only when the incubation in the caecum is long enough, allowing to CWE to increase the fermentation. This effect was more evident in the fermentation of SBM, suggesting that the crude protein (CP) content of substrates (515 vs. 388g/kg DM for SBM and SFM, respectively) could affect the kinetics of in vitro fermentation.
    Source: 9th World Rabbit Congress June 10-13, 2008 Verona (Str. 689-693)
    Type of material - conference contribution
    Publish date - 2008
    Language - english
    COBISS.SI-ID - 2325640

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