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Ultrasound in phage display: a new approach to nonspecific elutionLunder, Mojca, 1977- ...Libraries of phage-displayed random peptides are routinely used to identify target-binding peptides. Phages are commonly eluted in a nonspecific manner, especially if there are no available ligands ... of the particular target to use as competitors. However, the present study clearly demonstrates that nonspecific elution is not always able to break peptide-target interactions. To circumvent this we have developed an improved nonspecific elution strategy that uses ultrasound to release target-bound phages and enables selection of high-affinity clones in a single step.Source: Biotechniques. - ISSN 0736-6205 (Vol. 44, no. 7, 2008, str. 893-900)Type of material - article, component partPublish date - 2008Language - englishCOBISS.SI-ID - 2347377
Author
Lunder, Mojca, 1977- |
Bratkovič, Tomaž, farmacevt, 1978- |
Urleb, Uroš |
Kreft, Samo |
Štrukelj, Borut
Topics
bakteriofagna knjižnica |
peptidi |
ultrazvok
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Database name | Field | Year |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
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Lunder, Mojca, 1977- | 25096 |
Bratkovič, Tomaž, farmacevt, 1978- | 23598 |
Urleb, Uroš | 01878 |
Kreft, Samo | 15460 |
Štrukelj, Borut | 07849 |
Source: Personal bibliographies
and: SICRIS
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