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Erlihioza v Sloveniji: molekularno - epidemiološke značilnosti : doktorska disertacija = Ehrlichiosis in Slovenia: molecular and epidemiological characteristics : dissertation thesisPetrovec, MiroslavTick-transmitted diseases represents important public health problems in Slovenia. During the last decade, two previously unknown human diseases causedby Ehrlichia species have emerged in United ... States. During this study febrile ilnesses following a tick bite in patients from Slovenia were evaluated for an ehrlichial etiology. With molecular and serological methods we have confirmed first patients infected with granulocytic ehrlichia outside the United States. Among 130 evaluated patients included in prospective study we have found four acute cases of human granulocytic ehrlichiosis. We suggeststhat human granulocytic ehrlichiosis should be included in differrential diagnosis when evaluating patients with febrile illness after a tick-bite. By using PCR method we have successfully amplified two target regions of ehrlichiae in human patients, deer (Capreolus capreolus), red deer (Cervus elaphus), chamois (Rupicapra rupicapra), and small mammals (Chlethrionomys glareoulus, Apodemus flavicollis and Apodemus sylvaticus). PCRproducts were sequenced and sequences compared with those of known ehrlichiae. Comparison of GroESL operon sequences revealed that sequences fromSlovenian patients were more similar to the sequences obtained from animals in Europe than to sequences amplifed from patients in the United States. In Ixodes ricinus ticks we have indentified sequences 100 % identical with those amplified from patients. Therefore we conclude that I. ricinus is potential vector of granulocytic ehrlichia in Slovenia. In the first study in Slovenia the prevalence of infection among 906 Ixodes ricinus ticks was 9,2 % and 7,2 % for adults and nymphs, respectively. Phylogenetic analysis revealed that granulocytic ehrlichia amplified from patients has 100 % identical sequence in 16S rDNA with prototype human granulocytic ehrlichia from United States. (Abstract truncated at 2000 characters.)Type of material - dissertationPublication and manufacture - Ljubljana : [Biotehniška fakulteta] : [M. Petrovec], 1999Language - slovenianCOBISS.SI-ID - 2351736
Author
Petrovec, Miroslav
Other authors
Koren, Srečko |
Avšič-Županc, Tatjana
Topics
erlihioza |
prenašalci |
gostitelji |
klopi |
Ixodes ricinus |
epidemiologija |
epidemiologija |
ehrlichiosis |
vectors |
reservoirs |
ticks |
Ixodes ricinus |
epidemiology |
epidemiology
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Library/institution |
City | Acronym | For loan | Other holdings |
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MF, Central Medical Library, Ljubljana | Ljubljana | CMK |
outside loan 2 cop.
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BF, Central Biotechnical Library, Ljubljana | Ljubljana | BFCBK |
outside loan 1 cop.
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BF, Department of Food Science and Technology, Lj. | Ljubljana | BFZIV |
outside loan 1 cop.
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National and University Library, Ljubljana | Ljubljana | NUK |
reading room 1 cop.
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JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
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DRS, in which the journal is indexed
Database name | Field | Year |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
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Petrovec, Miroslav | 15902 |
Koren, Srečko | 07264 |
Avšič-Županc, Tatjana | 10331 |
Source: Personal bibliographies
and: SICRIS
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