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Spremljanje biosintezne stabilnosti plesni Mucor miehei pri proizvodnji ekstracelularnih proteaz : diplomska naloga = Stability of mould Mucor miehei for biosynthesis of extracelular proteases : graduation thesis
Hude, Stanislava
Namen naloge je spremljati biosintezno stabilnost plesni ▫{\sl M. miehei}▫ pri proizvodnji ekstracelularnih proteaz in ugotoviti vpliv Uv sevanja na sposobnost biosinteze koagulativnega encima. ...Stabilnost biosinteze smo testirali pri polisporni kulturi, monokulturah A, B, C in D, ki smo jih vzgojili iz polisporne kulture, in pri petih mutantih ▫{\sl M. miehei}▫ MB v različnihčasovnih intervalih. Ugotovili smo, da so delovne kulture stabilne pri biosintezi koagulativnega encima. Polisporna kultura dosega encimsko aktivnost 2265,9 E/ml do 2561,1 E/ml. Monokulture B, C in D so si po encimski aktivnosti zelo podobne. Vrednosti encimske aktivnosti se gibljejo od 2136,3 E/ml do 2270,1 E/ml. Odstopa monokultura A, ki ima zelo nizko encimsko aktivnost 100,1 E/ml. Po izvedbi mutacije dobimo mutante plesni ▫{\sl M. miehei}▫ MB, ki imajo izraženo biosintezno sposobnost produkcije koagulativnega encima.
Type of material - undergraduate thesis
; adult, serious
Publication and manufacture - Ljubljana : [S. Hude], 1996
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