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Micelarna elektrokinetična kromatografija za določanje aminokislin v vzorcih koruze : diplomska naloga = Micelar electrokinetic cromatography for analysis of amino acids in maize samples : graduation thesis
Stražišar, Monika
V vzorcih koruz različnih genotipov smo kvalitativno, semikvantivno ter kvantitativano določili vsebnost aminokislin. Pri pripravi vzorcev smo se poslužili standardne hidrolize s kislino (6N HCl, 24 ...ur, 120°C) ter derivatizacije z DNS-Cl (ker aminokisline slabo absorbirajo v območju UV in vidne svetlobe). Ločbo derivatov smo dosegli z micelarno elektrokinetično kromatografijo. Rezultati so potrdili že znana dejstva, da koruza vsebuje malo metionina (1,59-2,69 g/100 g beljakovin) in lizina (1,17-4,44 g/100 g beljakovin ter veliko leucina (18,87-24,48 g/100 g beljakovin). Poleg naštetih aminokislin smo kvalitativno določili še treonin, serin, alanin, prolin, glutaminsko kislino in glutamin, asparaginsko kislino in asparagin, glicin, valin, izoleucin, fenilalanin in arginin. Triptofana nismo mogli določiti, ker se je pri kisli hidrolizi razgradil. Razlike med vzorci so majhne. Metoda se je, tako z ekonomskega kot ekološkega stališča, izkazala kot primerna tehnika za ovrednotenje kakovosti živil.
Type of material - undergraduate thesis
; adult, serious
Publication and manufacture - Ljubljana : [M. Stražišar] : [BF, Oddelek za živilstvo], 1996
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