-
EAAT2 density at the astrocyte plasma membrane and Ca(2 + )-regulated exocytosisStenovec, Matjaž ...We studied whether regulated exocytosis affects the glutamate transporter density in cultured astrocytes, in which the expression of a fluorescently labeled excitatory amino acid transporter 2 ... (EAAT2-EGFP) predominantly labeledthe plasma membrane. The addition of ionomycin that elevates cytosolic Ca(2+) strongly increased the fluorescence of FM 4-64 membrane area dye, confirming the presence of regulated exocytosis in transfected astrocytes. However, concomitant with Ca(2+)-dependent FM 4-64 fluorescence increase, ionomycin induced a significant steady-state decrease in EAAT2-EGFP fluorescence. This is likely due to a secondary inner filter effect since,(i) in the absence of FM 4-64, ionomycin stimulation was ineffective in changing the EAAT2-EGFP fluorescence, and (ii) fluorescence changes in FM 4-64 and EAAT2-EGFP were inversely correlated. To test whether subcellular EAAT2-EGFP structures are translocated from the cytoplasm to the plasma membrane during ionomycin stimulation, EAAT2-EGFP fluorescence was monitored locally at the plasma membrane and a few microns away in the adjacent cytoplasm. Measurementsrevealed sites with an increase in EAAT2-EGFP plasma membrane fluorescence correlated with a fluorescence decrease beneath the plasma membrane, and sites with plasma membrane fluorescence decrease correlated withfluorescence increase within the adjacent cytoplasm. The sites of rapid translocation/retrieval of EAAT2-EGFP structures to/from the plasma membrane appeared to be distributed in a punctuate pattern around the cell perimeter. The density of EAAT2-EGFP was regulated in a Ca(2+)-dependent manner, since in the absence of extracellular Ca(2+) local translocation/retrieval events were absent, revealing rapid surface density regulation of EAAT2 in astrocytesby regulated exo/endocytosis.Source: Molecular membrane biology. - ISSN 0968-7688 (Letn. 25, št. 3, 2008, str. 203-215)Type of material - article, component partPublish date - 2008Language - englishCOBISS.SI-ID - 24138713
Author
Stenovec, Matjaž |
Kreft, Marko |
Grilc, Sonja |
Pangršič, Tina |
Zorec, Robert, 1958-
Topics
Astrocytes |
Exocytosis |
Calcium |
Membrane Proteins |
Cytosol |
Biological Transport, Active |
Ionomycin |
Cells, Cultured |
Microscopy, Confocal |
Eksocitoza |
Citosol |
Membranske beljakovine |
Kalcij |
Astrociti |
Biološki prenos aktivni |
Mikroskopija konfokalna |
Ionomicin |
Celične kulture
Shelf entry
Permalink
- URL:
Impact factor
Access to the JCR database is permitted only to users from Slovenia. Your current IP address is not on the list of IP addresses with access permission, and authentication with the relevant AAI accout is required.
Year | Impact factor | Edition | Category | Classification | ||||
---|---|---|---|---|---|---|---|---|
JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Select the library membership card:
DRS, in which the journal is indexed
Database name | Field | Year |
---|
Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
---|---|
Stenovec, Matjaž | 15467 |
Kreft, Marko | 15666 |
Grilc, Sonja | 06846 |
Pangršič, Tina | 23346 |
Zorec, Robert, 1958- | 03702 |
Select pickup location:
Material pickup by post
Notification
Subject headings in COBISS General List of Subject Headings
Select pickup location
Pickup location | Material status | Reservation |
---|
Please wait a moment.