ALL libraries (COBIB.SI union bibliographic/catalogue database)
PDF
  • Effects of chondrogenic and osteogenic regulatory factors on composite constructs grown using human mesenchymal stem cells, silk scaffolds and bioreactors
    Augst, Alexander ...
    Human mesenchymal stem cells (hMSCs) isolated from bone marrow aspirates were cultured on silk scaffolds in rotating bioreactors for three weeks with eitherchondrogenic or osteogenic medium ... supplements to engineer cartilage- or bone-like tissue constructs. Osteochondral composites formed from these cartilage and bone constructs were cultured for an additional three weeks in culture medium that was supplemented with chondrogenic factors, supplemented with osteogenic factors or unsupplemented. Progression of cartilage and bone formation and the integration between the two regions were assessed by medicalimaging (magnetic resonance imaging and micro-computerized tomography imaging), and by biochemical, histological and mechanical assays. During composite culture (three to six weeks), bone-like tissue formation progressed in all three media to a markedly larger extent than cartilage-like tissue formation. The integration of the constructs was most enhanced in composites cultured in chondrogenic medium. The results suggest that tissue composites with well-mineralized regions and substantially less developed cartilage regions can be generated in vitro by culturing hMSCs on silk scaffolds in bioreactors, that hMSCs have markedly higher capacity for producing engineeredbone than engineered cartilage, and that chondrogenic factors play major roles at early stages of bone formation by hMSCs and in the integrationof the two tissue constructs into a tissue composite.
    Source: Journal of the Royal Society interface. - ISSN 1742-5689 (Letn. 5, št. 25, 2008, str. 929-939)
    Type of material - article, component part
    Publish date - 2008
    Language - english
    COBISS.SI-ID - 24142041
    DOI

Shelf entry