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Opis ksilanolitičnih encimskih aktivnosti vampne bakterije Butyrivibrio sp. Mz5 : diplomska naloga = Characterisation of the xylanolytic enzyme activities of the rumen bacterium Butyrivibrio sp. Mz5 : graduation thesis
Čepeljnik, Tadej
Butirivibriji so ene izmed najpogostejǴih izoliranih bakterij iz vampa razliřnih preÍvekovalcev. Sev Butyrivibrio sp. Mz5 je bil prvotno izoliran iz vampa řrno-bele frizijske krave (Univerza v ...Ljubljani, BiotehniǴka fakulteta, Oddelek za zootehniko, Slovenija) in ima zelo mořno izraÍeno ksilanolitiřno aktivnost. Izmerili so, da je vsaj 1,65-krat veřja kot pri ostalih testiranih bakterijah. NajviǴja izmerjena na celice vezana ksilanolitiřna aktivnost je bila 5507 nmol RS/mg PROT/min. Na SDS PAGE-ksilanogramih smo pri vzorcih celiczaznali 5 jasno izraÍenih ksilanaz z molekulskimi masami 26,7 kDa, 51 kDa, 77,4 kDa, 99,8 kDa in 145 kDa. Poleg njih je bilo na ksilanogramih moÍno dokazati Ǵe 9 Ǵibkeje izraÍenih ksilanolitiřnih encimov (48 kDa, 53,2 kDa, 58 kDa, 82,3 kDa, 87,9 kDa, 104 kDa, 111,3 kDa, 126,6 kDa in 137 kDa). Nobena od ksilanaz ni řisti zunajceliřni encim. Njihovo izraÍanje je mořno inducirano s ksilanom in odvisno od faze rasti. Iz celiřnega izvleřka smo z anionsko izmenjavo in gelsko filtracijo delno izolirali dva ksilanolitiřna encima z molekulskima masama 51 in 58 kDa. Ksilanolitiřne lastnosti seva Mz5 kaÍejo na dobre moÍnosti za biotehnoloǴko uporabo seva in/ali njegovih izoliranih ksilanaz.
Type of material - undergraduate thesis
; adult, serious
Publication and manufacture - Ljubljana : [T. Čepeljnik] : [BF, Enota meddoddelčnega študija mikrobiologije], 2000
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