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Prevalence of toxin encoding genes in Escherichia coli isolates from urinary tract infections in Slovenia = Prevalenca genskih zapisov za toksine v izolatih bakterije Escherichia coli, pridobljenih iz vzorcev urina v SlovenijiStarčič Erjavec, Marjanca ...Methods 110 uropathogenic Escherichia coli strains (UPEC) obtained from the Institute of Microbiology and Immunology of the Medical Faculty in Ljubljana were screened by PCR with primers specific for ... the following toxin encoding genes: hlyA (haemolysin), cnf1 (cytotoxic necrotising factor 1), usp (uropathogenic specific protein USP) and ibeA (invasin). Dot blot hybridisation experiments were performed to validate the PCR assays. Results In 44% of the strains usp gene sequences were detected. The prevalence of hlyAand cnf1 was 25% and 23%, respectively. Only 9% of the strains harbored ibeA. The majority of the tested toxin encoding genes was found in strains belonging to the B2 phylogenetic group. Conclusions The toxin encoding genes hlyA, cnf1 and usp were strongly co-associated. Further, we found a statistically significant co-association of ibeA and usp. The prevalence of the tested toxin encoding genes in E. coli strains from urinary tract infections isolated in Slovenia is comparable to those from studies in other geographic regions.Source: Zdravniški vestnik : glasilo Slovenskega zdravniškega društva = Slovenian medical journal = journal of Slovenian Medical Association. - ISSN 1318-0347 (Letn. 77, št. 6-7, 2008, str. 427-432)Type of material - article, component partPublish date - 2008Language - englishCOBISS.SI-ID - 24527577
Author
Starčič Erjavec, Marjanca |
Križan-Hergouth, Veronika |
Gubina, Borut |
Žgur-Bertok, Darja
Topics
Urinary Tract Infections |
Microbiology |
Escherichia Coli |
Classification |
Genetics |
Bacterial Toxins |
Virulence |
Polymerase Chain Reaction |
Oligonucleotide Probes |
Polimerazna, verižna reakcija |
Escherichia coli |
Sečila, infekcije |
Bakterijski toksini |
Virulenca |
Oligonukleotidne sonde
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Database name | Field | Year |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
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Starčič Erjavec, Marjanca | 16064 |
Križan-Hergouth, Veronika | 10905 |
Gubina, Borut | 23790 |
Žgur-Bertok, Darja | 07042 |
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