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Innervation and electrical pulse stimulation - in vitro effects on human skeletal muscle cellsMarš, Tomaž, 1972- ...Contraction-induced adaptations in skeletal muscles are well characterized in vivo, but the underlying cellular mechanisms are still not completely understood. Cultured human myotubes represent an ... essential model system for human skeletal muscle which can be modulated ex vivo, but they are quiescent and do not contract unless being stimulated. Stimulation can be achieved by innervation of human myotubes in vitro by co-culturing with embryonic rat spinal cord, or by replacing motor neuron activation by electrical pulse stimulation (EPS). Effects of these two in vitro approaches, innervation and EPS, were characterized with respects to the expression of myosin heavy chains (MyHCs) and metabolism of glucose and oleic acid in cultured human myotubes. Adherent human myotubes were either innervated with rat spinal-cord segments or exposed to EPS. The expression pattern of MyHCs was assessed by qPCR, immunoblotting, and immunofluorescence, while the metabolism of glucose and oleic acid were studied using radiolabeled substrates. Innervation and EPS promoted differentiation towards different fiber types in human myotubes. Expression of the slow MyHC-1 isoform was reduced in innervated myotubes, whereas it remained unaltered in EPS-treated cells. Expression of both fast isoforms (MyHC-2A and MyHC-2X) tended to decrease in EPS-treated cells. Both approaches induced a more oxidative phenotype, reflected in increased CO2 production from both glucose and oleic acid. Novelty: Innervation and EPS favour differentiation into different fiber types in human myotubes. Both innervation and EPS promote a metabolically more oxidative phenotype in human myotubes.Source: Applied physiology, nutrition and metabolism = Physiologie appliquée, nutrition et métabolisme. - ISSN 1715-5312 (Vol. 46, no. 4, Apr. 2021, str. 299-308)Type of material - article, component partPublish date - 2021Language - englishCOBISS.SI-ID - 24641539
Author
Marš, Tomaž, 1972- |
Miš, Katarina |
Meznarič, Marija |
Prpar Mihevc, Sonja |
Jan, Vid |
Haugen, Fred |
Rogelj, Boris |
Rustan, Arild C. |
Thoresen, G. Hege |
Pirkmajer, Sergej |
Nikolić, Nataša, farmacevtka
Topics
skeletal muscles |
electrical pulse stimulation |
innervation |
skeletne mišice |
stimulacija električnega impulza |
inervacija
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Database name | Field | Year |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
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Marš, Tomaž, 1972- | 16345 |
Miš, Katarina | 19318 |
Meznarič, Marija | 04905 |
Prpar Mihevc, Sonja | 30710 |
Jan, Vid | 38241 |
Haugen, Fred | ![]() |
Rogelj, Boris | 15813 |
Rustan, Arild C. | ![]() |
Thoresen, G. Hege | ![]() |
Pirkmajer, Sergej | 28351 |
Nikolić, Nataša, farmacevtka | ![]() |
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