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  • Reciprocal neural regulation of extrajunctional acetylcholinesterase and collagen Q in rat muscles-The role of calcineurin signaling
    Trinkaus, Miha ; Pregelj, Peter ; Sketelj, Janez
    There are two main differences regarding acetylcholinesterase (AChE) expression in the extrajunctional regions of fast and slow rat muscles: (1) the activity of AChE catalytic subunits (G1 form) is ... much higher in fast thanin slow muscles, and (2) the activity of the asymmetric forms of AChE (A(8) and A(12)) is quite high extrajunctionally in slow muscles but virtuallyabsent in fast muscles. The latter is due to the absence of the expression of AChE-associated collagen Q (ColQ) in the extrajunctional regionsof fast muscle fibers, in contrast to its ample expression in slow muscles. We showed that both differences are caused by different neural activation patterns of fast vs. slow muscle fibers, which determine the respective levels of mRNA of both proteins. Whereas the changes in AChE mRNA levels in fast and slow muscles, as well as the levels of ColQ mRNA levels in slow muscles, observed in response to exposing either slow or fast muscles to different muscle activation patterns, are completely reversible, the extrajunctional suppression of ColQ expression in fast muscle fibers seems to be irreversible. Calcineurin signaling pathway in muscles is activated by high-average sarcoplasmic calcium concentration resulting from tonic low-frequency muscle fiber activation pattern, typical for slow muscle fibers,but is inactive in fast muscle fibers, which are activated by infrequent high-frequency bursts of neural impulses. Application to rats of two inhibitors of calcineurin (tacrolimus-FK506 and cyclosporin A) demonstrated that the mRNA levels of both the AChE catalytic subunit and ColQ in the extrajunctional regions of the soleus muscle are regulated by the calcineurin signaling pathway, but in a reciprocal way. Under the conditions of low calcineurin activity, AChE expression is enhanced and that of ColQ is suppressed, and vice versa. (Abs. trunc. at 2000 ch.)
    Source: Chemico-biological interactions. - ISSN 0009-2797 (Letn. 175, št. 1/3, 2008, str. 45-49)
    Type of material - article, component part
    Publish date - 2008
    Language - english
    COBISS.SI-ID - 24742361
    DOI

source: Chemico-biological interactions. - ISSN 0009-2797 (Letn. 175, št. 1/3, 2008, str. 45-49)
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