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Intracytoplasmic morphologically selected sperm injection improves developmentand quality of preimplantation embryos in teratozoospermia patientsKnez, Katja, 1985- ...This prospective randomized study investigated whether intracytoplasmic sperm injection (ICSI) outcome can be improved with sperm preselection under Ž6000 magnification and intracytoplasmic ... morphologically selected sperm injection (IMSI) in patients with teratozoospermia and characterized embryo development and quality regarding sperm morphology and presence of head vacuoles. Couples with isolated teratozoospermia were divided into two groups: IMSI group (n = 52) and ICSI group (n = 70) and fertilization, blastocyst and clinical pregnancy rates were compared. Oocytes from 30 randomly chosen patients from the IMSI group were injected with spermatozoa that had been previously classified under Ž6000 magnification into four classes according to the numberand size of vacuoles in the head and then cultured separately. Pronuclear morphology, embryo development and blastomere viability were estimated to investigate the influence of sperm morphology, especially vacuoles, on embryo developmental capacity. A significantly higher clinical pregnancy rate was achieved in the IMSI group compared with the ICSI group (48% versus 24%, P < 0.05). Fertilization with spermatozoa without head vacuoles yielded higher number of morphologically normal zygotes, higher blastocyst rate and smaller proportion of arrested embryos than spermatozoa with vacuoles and other head defects. IMSI is a method of choice in patients with teratozoospermia.Source: Reproductive biomedicine online. - ISSN 1472-6483 (Vol. 25, iss. 2, Avg. 2012, str. 168-179)Type of material - article, component part ; adult, seriousPublish date - 2012Language - englishCOBISS.SI-ID - 249772
Author
Knez, Katja, 1985- |
Tomaževič, Tomaž |
Zorn, Branko |
Vrtačnik-Bokal, Eda |
Virant-Klun, Irma
Topics
blastomere viability |
IMSI |
paternal effect |
pronuclear morphology |
tetrazoospermia |
vacuole |
vacuole
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source: Reproductive biomedicine online. - ISSN 1472-6483 (Vol. 25, iss. 2, Avg. 2012, str. 168-179)
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Database name | Field | Year |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
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Knez, Katja, 1985- | ![]() |
Tomaževič, Tomaž | 07007 |
Zorn, Branko | 19449 |
Vrtačnik-Bokal, Eda | 12177 |
Virant-Klun, Irma | 06087 |
Source: Personal bibliographies
and: SICRIS
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