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Učinek lokalnega anestetika na preverjanje po senzoričnem živcu podgane v različnih poskusnih razmerah = Effect of local anesthetic solution on rat sensory nerve conduction in different experimental conductions : doktorska disertacija
Potočnik, Igor, 1965-
Blockade of the inferior alveolar nerve is the single most commonly desired goal of mandibular anesthesia. Difficulty, experienced in obtaining satisfactory analgesia of lower molars after the ...standard approach (inferior alveolar nerve block, IANB), remains a common clinical problem. Recent research of reasons for failure of IANB is focused on the effect of local anesthetic solution on peripheral nerve conduction. The aim of the present study was to examine the effect of different types of anesthetic solutions, critical length of the nerve exposed to anesthetic solution, inflammation of innervated tissue, age and gender, on sensitivity of different classes of peripheral sensory nerve fibers. The basic approach was to examine changes in compound action potential (CAP) of A and C fibers of the rat sural nerve, assessed by electrophysiological method in vitro. Nerve block was induced by placing longitudinally dissected, 3mm (6 and 9 mm) long silicon tube around the nerve segment between stimulating and recording electrodes, and injection 0,1 ml (0,2 and 0,3 ml) of anesthetic solution into the tube volume. Before and after nerve block induction, surface, amplitude, latency, duration and time taken to develop maximal depression of CAP, evoked by suprathreshold stimuli, were recorded. The CAPs recorded from sural nerves following suprathreshold electrical stimulation before lidocaine application revealed three distinct groups of waves corresponding to the Aaž(3, A8 and C fibers. Inall experimental conditions, C fibers were blocked approximatelly 30 secondsafter application of anesthetic solution. There were no significant differences between experimental groups. In contrast, application of any tested anesthetic solution have not resulted in total nerve block of myelinated fibers. (Abstract truncated at 2000 characters).
Type of material - dissertation
Publication and manufacture - Ljubljana : [I. Potočnik], 2003
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