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Sensitivity of intergenic regions of yeast mitochondrial DNA to single-strand-specific nucleasesPiškur, Jure, 1960-2014The reactivity of mitochondrial DNA (mtDNA) sequences from Torulopsis glabrataand Saccharomyces cerevisiae towards single-strand-specific nucleases has been examined. AT-rich stretches located in ... intergenic sequences from bothyeasts were cleaved by nucleases when the sequences were contained in supercoiled plasmid DNA. In particular ori/rep sequences from the mtDNA of S. cerevisiae were shown to be sensitive to the single-strand-specific nucleases.The locations of the sensitive sites were related to the organisation of the sequence domains of ori/rep and the superhelicity of the DNA, as well as the presence of particular sequences. It is proposed that distortions of the DNA duplex could be generated in mtDNA molecules in vivo and that these distortions may provide a substrate for enzymes involved in transmission, recombination and/or transcription of mtDNA.Source: FEBS letters. - ISSN 0014-5793 (Vol. 238, no. 2, 1988, str. 435-440)Type of material - article, component partPublish date - 1988Language - englishCOBISS.SI-ID - 25495513
Author
Piškur, Jure, 1960-2014
Topics
Candida |
Genetics |
Dna Restriction Enzymes |
DNA, Fungal |
Genetics |
Metabolism |
DNA, Mitochondrial |
Genetics |
Metabolism |
DNA, Superhelical |
Metabolism |
Deoxyribonucleases |
Metabolism |
Endonucleases |
Metabolism |
Ethidium |
Pharmacology |
Plasmids |
Saccharomyces Cerevisiae |
Genetics |
Saccharomyces cerevisiae |
DNA superheliksna |
DNA mitohondrijska |
DNA restrikcijski encimi |
Deoksiribonukleaze |
Plazmidi |
DNA glivična |
Kandida |
Endonukleaze |
Etidij
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Piškur, Jure, 1960-2014 | 31275 |
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