ALL libraries (COBIB.SI union bibliographic/catalogue database)
-
Usage of the standard and modified comet assay in assessment of DNA damage in human lymphocytes after exposure to ionizing radiation = [Primerjava standardnega in modificiranega testa komet pri ugotavljanju poškodb DNA na humanih limfocitih po izpostavitvi ionizirajočemu sevanju]Mikloš, Morana ; Gajski, Goran ; Garaj Vrhovac, VericaBackground. Human organisms are extremely sensitive to ionizing radiation, which has the strong genotoxic effect on the DNA molecule. The aim of the present study was to detect the type of DNA damage ... and cell death caused by ionizing radiation as well as the sensitivity of the standard and modified comet assay. Methods. The effect of gamma radiation (0.1 Gy and 4 Gy) on humanlymphocytes was observed using the standard alkaline and Fpg-modified comet assay with ability to detect oxidized purines as well as with the DNA diffusion test. Results. Parameters of the standard comet assay showed significantly higher values in samples exposed to 4 Gy than in samples exposed to radiation dose of 0.1 Gy and control sample. The Fpg-modified cometassay showed significantly higher values already at dose of 0.1 Gy as theresult of oxidative DNA damage. The DNA diffusion test showed that gamma rays lead to apoptosis more often than to necrosis. Conclusions. This observation suggests that the standard alkaline and Fpg-modified comet assays as well as the DNA diffusion test are reliable techniques for estimation of DNA damage and form of the cell death caused by gamma radiation in vitro. In addition Fpg-modified comet assay prove to be more sensitive for detection of gamma irradiation induced DNA damage than the standard assay.Source: Radiology and oncology. - ISSN 1318-2099 (Vol. 43, no. 2, 2009, str. 97-107)Type of material - article, component part ; adult, seriousPublish date - 2009Language - englishCOBISS.SI-ID - 25672665
Author
Mikloš, Morana |
Gajski, Goran |
Garaj Vrhovac, Verica
Topics
Lymphocytes |
Radiation Effects |
DNA Damage |
Electrophoresis, Agar Gel |
Elektroforeza, agar gel |
Limfociti |
DNA, poškodba
Shelf entry
Permalink
- URL:
Impact factor
Access to the JCR database is permitted only to users from Slovenia. Your current IP address is not on the list of IP addresses with access permission, and authentication with the relevant AAI accout is required.
Year | Impact factor | Edition | Category | Classification | ||||
---|---|---|---|---|---|---|---|---|
JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Select the library membership card:
If the library membership card is not in the list,
add a new one.
DRS, in which the journal is indexed
Database name | Field | Year |
---|
Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
---|---|
Mikloš, Morana | |
Gajski, Goran | |
Garaj Vrhovac, Verica |
Source: Personal bibliographies
and: SICRIS
Select pickup location:
Material pickup by post
Delivery address:
Address is missing from the member's data.
The address retrieval service is currently unavailable, please try again.
By clicking the "OK" button, you will confirm the pickup location selected above and complete the reservation process.
By clicking the "OK" button, you will confirm the above pickup location and delivery address, and complete the reservation process.
By clicking the "OK" button, you will confirm the address selected above and complete the reservation process.
Notification
Automatic login and reservation service currently not available. You can reserve the material on the Biblos portal or try again here later.
Subject headings in COBISS General List of Subject Headings
Select pickup location
The material from the parent unit is free. If the material is delivered to the pickup location from another unit, the library may charge you for this service.
Pickup location | Material status | Reservation |
---|
Reservation in progress
Please wait a moment.
Reservation was successful.
Reservation failed.
Reservation...
Membership card:
Pickup location: