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The riddle of mitochondrial caspase-3 from liverMilisav, Irina ; Nipič, Damijan ; Šuput, DušanCaspase-3 is one of the main executors of apoptosis. Its zymogen procaspase-3 was localized to cytosol, mitochondria and nuclei. The subcellular location ofprocaspase-3 in liver was reported by ... several studies to be either cytosolicor cytosolic and mitochondrial. Our aim was to investigate these separate procaspase-3 pools to differentiate the pathways of their activation.By cell fractionation, immunocytochemistry, and confocal microscopywe report that there is a single procaspase-3 pool located to the cytosol in primary hepatocytes and in fractions of rat liver. In contrast, it depends on the isolation purity whether procaspase-3 is located in mitochondria of non-parenchymal liver cells, or not. All preparations with mitochondrial procaspase-3 fractions contain traces of haemoglobin, indicatingthe presence of some erythrocytes, which are the source of mitochondrial procaspase-3. Since erythrocytes migrate with mitochondria in subcellular fractionations, it is important to check for haemoglobin, before localizing the protein to mitochondria.Source: Apoptosis. - ISSN 1360-8185 (Letn. 14, št. 9, 2009, str. 1070-1075)Type of material - article, component partPublish date - 2009Language - englishCOBISS.SI-ID - 25835993
Author
Milisav, Irina |
Nipič, Damijan |
Šuput, Dušan
Topics
Mitochondria, Liver |
Chemistry |
Apoptosis |
Cytosol |
Cell Fractionation |
Immunohistochemistry |
Microscopy, Confocal |
Hemoglobins |
Erythrocytes |
Cells, Cultured |
Citosol |
Celica, frakcioniranje |
Imunohistokemija |
Mitohondriji jetrni |
Apoptoza |
Hemoglobini |
Mikroskopija konfokalna |
Eritrociti |
Celične kulture
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Database name | Field | Year |
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Milisav, Irina | 18825 |
Nipič, Damijan | 28326 |
Šuput, Dušan | 07002 |
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