-
Functional characterization of N-terminally GFP-tagged GLP-1 receptorBavec, Aljoša ; Ličar, AlenkaThe glucagon-like peptide-l receptor (GLP-l receptor) mediates important effects on peripheral tissues and the central nervous system. It seems one of the most promising therapeutic targets for ... treatment of diabetes mellitus type2. Surprisingly, very little is known about the cellular mechanisms that regulate its function in vivo. One of the approaches to study receptor dynamics, expression, or signaling is using GFP-tagged fluorescent proteins. In this study, we synthesized and characterized N-terminally GFP-tagged GLP-l (GFP-GLP-l) receptor in eHO cells. We demonstrated that GFP-GLP-l receptor is weakly expressed in the plasma membranes and is functionally coupled to adenylyl cyclase via heterotrimeric G-proteins, similarly as its wild type.Source: Journal of Biomedicine and Biotechnology (Online) [Elektronski vir]. - ISSN 1110-7251 (Št. 498149, 2009, str. 1-10)Type of material - e-articlePublish date - 2009Language - englishCOBISS.SI-ID - 26132697
Author
Bavec, Aljoša |
Ličar, Alenka
Topics
Cho Cells |
Receptors, Glucagon |
Peptides |
Adenyl Cyclase |
Recombinant Proteins |
Protein Binding |
Binding Sites |
Cyclic Amp |
Microscopy, Confocal |
Immunoblotting |
Diabetes Mellitus, Non-Insulin-Dependent |
CHO celice |
Rekombinantne beljakovine |
Receptorji glukagonski |
Peptidi |
Beljakovine, vezava |
Imunoblot |
Mikroskopija konfokalna |
Sladkorna bolezen, ne-inzulin-odvisna |
Mesta vezave |
Ciklični AMP
![loading ... loading ...](themes/default/img/ajax-loading.gif)
![loading ... loading ...](themes/default/img/ajax-loading.gif)
![loading ... loading ...](themes/default/img/ajax-loading.gif)
![loading ... loading ...](themes/default/img/ajax-loading.gif)
Shelf entry
Permalink
- URL:
Impact factor
Access to the JCR database is permitted only to users from Slovenia. Your current IP address is not on the list of IP addresses with access permission, and authentication with the relevant AAI accout is required.
Year | Impact factor | Edition | Category | Classification | ||||
---|---|---|---|---|---|---|---|---|
JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Select the library membership card:
DRS, in which the journal is indexed
Database name | Field | Year |
---|
Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
---|---|
Bavec, Aljoša | 16130 |
Ličar, Alenka | 29033 |
Select pickup location:
Material pickup by post
Notification
Subject headings in COBISS General List of Subject Headings
Select pickup location
Pickup location | Material status | Reservation |
---|
Please wait a moment.