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Ultrasound elution of biological ligands : patentna prijava : WO 2009007463 (A1), 2009-01-15
Lunder, Mojca, 1977- ...
The present invention discloses a method for analyzing the binding of a biological ligand to a target, comprising the steps of (1) providing one, two,or more different candidates for a biological ...ligand, (2) contacting said candidate(s) with a target, wherein for each candidate the target is the same or has the same structure, (3) applying ultrasound to the target- bound candidates for a ligand. In preferred embodiments, the invention relates to the use of the invention for screening biological libraries and in the contextof phage display. Preferably, the ultrasound is applied at acidic pH. The ultrasound may be applied during an elution and/or a wash step. The candidate for a biological ligand is preferably chosen from the group of peptides, peptidomimetics, antibodies, nucleic acids, carbohydrates, saccharides, oligosaccharides, polysaccharides, and glycosylated peptides. Furthermore, the present invention discloses the use of ultrasound during an elution and/or washing step in the screening of a biological library and the use of the method of the invention in a high-throughput screening.
Type of material - patent application
; adult, serious
Publication and manufacture - München : World Intellectual Property Organization, International Bureau, 2009
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