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Recombinant allergen-based IgE testing to distinguish bee and wasp allergyMittermann, Irene ...Bacground: The identification of the disease-causing insect in venom allergy is often difficult. Objective: To establish recombinant allergen-based IgE tests to diagnose bee and yellow jacket wasp ... allergy. Methods: Sera from patients with bee and/or wasp allergy (n = 43) and patients with pollen allergy with false-positive IgE serology to venom extracts were tested for IgEreactivity in allergen extract-based tests or with purified allergens, including nonglycosylated Escherichia coli-expressed recombinant (r) Api m 1, rApi m 2, rVes v 5, and insect cell-expressed, glycosylated rApi m 2 as well as 2 natural plant glycoproteins (Phl p 4, bromelain). Results: The patients with venom allergy could be diagnosed with a combination of E coli-expressed rApi m 1, rApi m 2, and rVes v 5 whereas patients with pollen allergy remainednegative. For a group of 29 patients for whom the sensitizing venom could not be identified with natural allergen extracts, testing with nonglycosylated allergens allowed identification of the sensitizing venom. Recombinant nonglycosylated allergens also allowed definition of the sensitizing venom for those 14 patients who had reacted either with bee or wasp venom extracts. By IgE inhibition studies, it is shown that glycosylated Api m 2 contains carbohydrate epitopes that cross-react with natural Api m 1, Ves v 2, natural Phl p 4, and bromelain, thus identifying cross-reactive structures responsible for serologic false-positive test results or double-positivity to bee and wasp extracts. Conclusions: Nonglycosylated recombinant bee and wasp venom allergens allow the identification of patients with bee and wasp allergy and should facilitate accurate prescription of venomimmunotherapy.Source: The Journal of allergy and clinical immunology. - ISSN 0091-6749 (Letn. 125, št. 6, 2010, str. 1300-1307)Type of material - article, component part ; adult, seriousPublish date - 2010Language - englishCOBISS.SI-ID - 27138521
Author
Mittermann, Irene |
Zidarn, Mihaela |
Šilar, Mira |
Markovic-Housley, Zora |
Aberer, Werner |
Korošec, Peter, 1970- |
Košnik, Mitja |
Valenta, Rudolf, 1963-
Topics
Bee Venoms |
Wasp Venoms |
Pollen |
Hypersensitivity, Immediate |
Diagnosis |
Allergens |
Ige |
Recombinant Proteins |
Escherichia Coli |
Transfection |
Cross Reactions |
Preobčutljivost takojšnja |
Alergeni |
Čebelji strupi |
Osji strupi |
Cvetni prah |
Rekombinantne beljakovine |
Escherichia coli |
Navskrižne reakcije |
IgE |
Transfekcija
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Database name | Field | Year |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
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Mittermann, Irene | ![]() |
Zidarn, Mihaela | 25317 |
Šilar, Mira | 25331 |
Markovic-Housley, Zora | ![]() |
Aberer, Werner | ![]() |
Korošec, Peter, 1970- | 22807 |
Košnik, Mitja | 10921 |
Valenta, Rudolf, 1963- | ![]() |
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