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  • Disturbed expression of phases I and II estrogen-metabolizing enzymes in endometrial cancer : lower levels of CYP1B1 and increased expression of S-COMT
    Hevir-Kene, Neli ; Šinkovec, Jasna, 1944- ; Lanišnik-Rižner, Tea
    Expression levels of genes encoding phases I and II estrogen-metabolizing enzymes: CYP1A1, CYP1A2, CYP1B1, CYP3A5, CYP3A7, SULT1A1, SULT1E1, SULT2B1, COMT, UGT2B7, and GSTP1 were studied by real-time ... PCR in 38 samples of cancerous and adjacent control endometrium. We found significantly lower levels of CYP1B1 and CYP3A7, higher levels of SULT2B1, UGT2B7 and GSTP1, and no differences in expression of COMT, CYP1A1, CYP3A5, SULT1E1 and SULT2A1 in the endometrial cancers. The CYP1B1 and COMT proteins were also examined by Western blotting and immunohistochemical staining, supporting the real-time PCR analysis. Lower levels of CYP1B1 detected in cancerous endometrium suggestits important role in control, precancerous tissue. Additionally, we showed for the first time higher protein levels of soluble COMT in cancerous endometrium, and higher levels of membrane-bound COMT in control, precancerousendometrium. The importance of the changed ratio between soluble and membrane-bound COMT still needs to be evaluated in further studies.
    Source: Molecular and cellular endocrinology. - ISSN 0303-7207 (Vol. 331, issue 1, 2011, str. 158-167)
    Type of material - article, component part
    Publish date - 2011
    Language - english
    COBISS.SI-ID - 27490009