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CD133/prominin1 is prognostic for GBM patient's survival, but inversely correlated with cysteine cathepsine' expression in gliobastoma derived spheroids = [CD133/prominin1 je prognostičen za preživetje bolnikov z glioblastoma, vendar obratno korelira z izražanjem cisteinskih katepsinov]Ardebili, Seyed Yousef ...Introduction. CD133 is a marker for a population of glioblastoma (GBM) and normal neural stem cells (NNSC). We aimed to reveal whether the migratory potential and differentiation of these stem cells ... is associated with CD133 expression and with cathepsin proteases (Cats). Materials and methods. The invasiveness of normal NNSC, GBM/CD133+ cell lines and GBM spheroids was evaluated in 3D collagen, as well as of U87-MG and normal astrocytes (NHA) grown in monolayers in 2D Matrigel. Expression of Cats B, L and S was measuredat mRNA and activity levels and their relation to invasiveness, to CD133 mRNA in 26 gliomas, and to the survival of these patients. Results. The average yield of CD133+ cells from GBM samples was 9.6%. Survival of patients with higher CD133 mRNA expression was significantly shorter (p< 0.005). Invasion, associated with proteolytic degradation of matrix, was higher in normal stem cells and GBM spheroids and cells than in isolated GBM CD133+ cells. In glioma samples, there was no correlation between CD133 mRNA expression and Cat mRNAs, but there was an inverse correlation with Cat activities. Conclusions. The study confirms CD133 as a prognostic marker for the survival of GBM patients. We demonstrated that NNSC have higher invasion potential and invade the collagen matrix in a mode different from that of GBM,initiating stem cell spheres. This result could have implications for the design of new therapeutics, including protease inhibitors that specifically target invasive tumour stem cells. Increased activity of cathepsins in CD133- cells suggests their role in the invasive behaviour of GBM.Source: Radiology and oncology. - ISSN 1318-2099 (Vol. 45, no. 2, 2011, str. 102-115)Type of material - article, component partPublish date - 2011Language - englishCOBISS.SI-ID - 28580825
Author
Ardebili, Seyed Yousef |
Zajc, Irena |
Gole, Boris, 1976- |
Campos, Benito |
Herold-Mende, Christel |
Drmota Prebil, Sara |
Lah Turnšek, Tamara
Topics
Glioblastoma |
Pathology |
Stem Cells |
Tumor Stem Cells |
Cathepsins |
Cysteine Proteinases |
Astrocytes |
RNA, Messenger |
Polymerase Chain Reaction |
Survival Analysis |
Matične celice |
Katepsini |
Glioblastom |
Tumorske matične celice |
RNA, prenašalna |
Polimerazna, verižna reakcija |
Preživetje, analiza |
Astrociti
Author | Ardebili, Seyed Yousef ... |
Title | CD133/prominin1 is prognostic for GBM patient's survival, but inversely correlated with cysteine cathepsine' expression in gliobastoma derived spheroids = [CD133/prominin1 je prognostičen za preživetje bolnikov z glioblastoma, vendar obratno korelira z izražanjem cisteinskih katepsinov] |
Publication date | 2011-06-03 |
COBISS.SI-ID | 28580825 |
Publication version in repository | Publisher's version |
Publication licence | Creative Commons Attribution 4.0 International |
Embargo | Immediate publication for public |
Project(s) from which the publication was funded
Title | Acronym | Project ID | Funder |
---|---|---|---|
Ekotoksiologija, toksikološka genomika in karcinogeneza | P1-0245-2009 |
Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije |
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Ardebili, Seyed Yousef | |
Zajc, Irena | 10974 |
Gole, Boris, 1976- | 26010 |
Campos, Benito | |
Herold-Mende, Christel | |
Drmota Prebil, Sara | 33160 |
Lah Turnšek, Tamara | 07802 |
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