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Effect of different sources of dietary fibre on oxidative stress in laboratory rats
Trebušak, Tina ...
The objective of the present study was to determine the effect of different sources of dietary fibre on the oxidative stress induced by a high fat diet in laboratory rats. Thirty two laboratory rats ...were penned individually and divided into four groups: CONT (high fat diet), G(70 g guar gum/kg), P(70 g apple pectin/kg) and WB(155 g wheat/kg). After 11 or 13 days of treatment DNA damage of blood leukocytes was measured by Comet assay and lipid peroxidation was studied by determining the malondialdehyde (MDA) concentration in liver and in urine. In comparison with group CONT, the degree of DNA damage was significantly lower in group WB. In groups G and P DNA damage was also reduced but not significantly. Similae results were also obtained for the liver MDA concentration. All three studied groups showed reduced liver MDA concentrations but only group WB was significant compared to group CONT. In comparison with group CONT, the groups WB and P had significantly reduced 24-hour urine MDA excretion, but not group G. The results of the experiment confirmed that wheat bran intake effectively reduces oxidative stress induced by a high fat diet.
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