ALL libraries (COBIB.SI union bibliographic/catalogue database)
-
Node optimisation in the three-node Turing genetic circuit incorporated in Escherichia coli = Optimizacija vozlišča v izbranem tri-vozliščnem Turingovem genetskem vezju, vgrajenem v Escherichia coli : magistrski študijski program Laboratorijska biomedicinaBahun, Nina, farmacevtkaIn 1952 Alan Turing published his seminal paper called >>The Chemical Basis of Morphogenesis<< in which he presents a reaction-diffusion model of biological pattern formation. Based on the ... interactions of two morphogens, a slowly diffusing activator and a fast diffusing inhibitor, this model enables the formation of spatial patterns which closely resemble those on the leopard spots, zebra stripes, and many other patterns found in nature. Turing patterns have been observed in different chemical systems, whilst their engineering in synthetic biology still presents a big challenge. Synthetic engineering of Turing patterns would present a big step forward in synthetic biology and would provide a better explanation for how patterning occurs in biology. It can also provide practical information for synthetic tissue production, the use of which could contribute to future therapeutic purposes. Previously, different types of genetic circuits capable of Turing patterns production were analysed computationally, and the '3954' Turing circuit proved most promising. This genetic circuit was previously implemented in E. coli chassis with protocatechuic acid (3,4-dihydroxybenxoic acid; PCA) and salicylic acid (2-hydroxybenzoic acid; SA) as node A diffusers. Most of the circuit components, such as promoters, synthesis enzymes, degradation enzymes and transcription factors, were previously optimised. The focus of this study was node A optimisation, which was performed by creating three different variants of node A, obtained by molecular cloning. These were then tested to verify if node A is functioning as expected. Based on the results obtained in this study, expression levels from node A are believed to be optimized, and all of its components are functioning correctly.Type of material - master's thesis ; adult, seriousPublication and manufacture - Ljubljana : [N. Bahun], 2020Language - englishCOBISS.SI-ID - 29108483
Author
Bahun, Nina, farmacevtka
Other authors
Lunder, Mojca, 1977- |
Isalan, Mark
Topics
Sintezna biologija |
Turingovi vzorci |
model reakcije in difuzije |
genetsko vezje '3954'
Library/institution |
City | Acronym | For loan | Other holdings |
---|---|---|---|---|
Faculty of Pharmacy, Ljubljana | Ljubljana | FFALJ |
reading room 1 cop.
|
Shelf entry
Permalink
- URL:
Impact factor
Access to the JCR database is permitted only to users from Slovenia. Your current IP address is not on the list of IP addresses with access permission, and authentication with the relevant AAI accout is required.
Year | Impact factor | Edition | Category | Classification | ||||
---|---|---|---|---|---|---|---|---|
JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Select the library membership card:
If the library membership card is not in the list,
add a new one.
DRS, in which the journal is indexed
Database name | Field | Year |
---|
Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
---|---|
Bahun, Nina, farmacevtka | |
Lunder, Mojca, 1977- | 25096 |
Isalan, Mark |
Source: Personal bibliographies
and: SICRIS
Select pickup location:
Material pickup by post
Delivery address:
Address is missing from the member's data.
The address retrieval service is currently unavailable, please try again.
By clicking the "OK" button, you will confirm the pickup location selected above and complete the reservation process.
By clicking the "OK" button, you will confirm the above pickup location and delivery address, and complete the reservation process.
By clicking the "OK" button, you will confirm the address selected above and complete the reservation process.
Notification
Automatic login and reservation service currently not available. You can reserve the material on the Biblos portal or try again here later.
Subject headings in COBISS General List of Subject Headings
Select pickup location
The material from the parent unit is free. If the material is delivered to the pickup location from another unit, the library may charge you for this service.
Pickup location | Material status | Reservation |
---|
Reservation in progress
Please wait a moment.
Reservation was successful.
Reservation failed.
Reservation...
Membership card:
Pickup location: