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Active protein aggregates produced in Escherichia coli [Elektronski vir]Peternel, Špela ; Komel, RadovanSince recombinant proteins are widely used in industry and in research, the need for their low-cost production is increasing. Escherichia coli is one of the best known and most often used host ... organisms for economical protein production. However, upon over-expression, protein aggregates called inclusionbodies (IBs) are often formed. Until recently IBs formation represented a bottleneck in protein production as they were considered as deposits of inactive proteins. However, recent studies show that by choosing the appropriate host strain and designing an optimal production process, IBs composed from properly folded and biologically active recombinant proteins canbe prepared. Such active protein particles can be further used for the isolation of pure proteins or as whole active protein particles in various biomedical and other applications. Therefore interest in understanding the mechanisms of their formation as well as their properties is increasing.Source: International journal of molecular sciences [Elektronski vir]. - ISSN 1422-0067 (Vol. 12, 2011, str. 8275-8287)Type of material - e-articlePublish date - 2011Language - englishCOBISS.SI-ID - 29115609
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Peternel, Špela | 22580 |
Komel, Radovan | 06135 |
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