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  • Keratinocyte-based cell assays : their potential pitfalls
    Zupančič, Tina, 1982- ...
    As an in vitro model system, patient-derived epidermolysis bullosa simplex keratinocytes have had an immense impact on what we know today about keratin filament function and their role in disease ... development. In the absence of gene therapy, screening com pound libraries for new or better drug s is another approach to improve existing treatments for genodennatoses. However inthis study, we report of the potential pitfalls when using this type of celllines as a "reporter" system. When cell lines with different genetic backgrounds are being used in cellbased assays, the greatest obstacle is to determine the most appropriate culture conditions (i.e., the composition of medium, number of cells plated and number of day s in culture). We demonstratehow culture conditions can greatly interfere with the cellular response in cell-based assays (cell proliferation, metabolic activity and migration), potentially al so giving rise to misleading data.
    Source: Archives for dermatological research. - ISSN 0340-3696 (Vol. 304, iss. 9, 2012, str. 765-768)
    Type of material - article, component part ; adult, serious
    Publish date - 2012
    Language - english
    COBISS.SI-ID - 30115545
    DOI