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  • Magnetic hydrophilic poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate microspheres for DNA isolation from faeces
    Trachtová, Štěpánka ...
    Magnetic non-porous hydrophilic poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate)-P(HEMA-co-GMA) microspheres containing carboxyl groups were used for DNA isolation from mouse faeces spiked ... by a probiotic Lactobacillus gasseri K7 strain. The quality of isolated DNA and the presence of traget DNA were verified by PCR and real time PCR using priomers specific for Lactobacillus genus or primers targeting gassericin A gene of the Lactobacillus gasseri K7 strain. For comparison, two other DNA extraction procedures were used. It was shown that DNA extracted by carboxylcoated P(HEMA-co-GMA) microspheres were sufficient for the amplification of target DNA using PCR and real-time PCR.
    Source: Proceedings of the 11th international conference on frontiers of polymers and advanced materials (ICFPAM 2011) (Vol. 555, issue 1, 2012, str. 263-270)
    Type of material - conference contribution
    Publish date - 2012
    Language - english
    COBISS.SI-ID - 3012744
    DOI

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