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  • Setting up a gene expression study for tissue cells by method of quantitative real-time PCR
    Dušanić, Daliborka ...
    Gene expression analysis by quantitative reverse transcription polymerase chain reaction (RT-qPCR) allows accurate and sensitive measurment of gene expression levels. However, a series of steps needs ... to be taken to ensure the relevance, accuracy, correct interpretation and repeatability of the RT-qPCR experiment. We describe here a simple experiment of determining relative gene expression for caspase 8 gene in chicken chondrocytes treated with an apoptosis inducing compound 5-fluorouracil. We use this example to point out some important guidelines in setting up a gene expression study in tissue cells, analyzing and interpreting the results and reporting on the findings.
    Source: Acta agriculturae Slovenica. - ISSN 1581-9175 (Letn. 100, št. 1, 2012, str. 19-28)
    Type of material - article, component part
    Publish date - 2012
    Language - english
    COBISS.SI-ID - 3081864