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Insertion of a specific fungal 3'-phosphoadenosine-5'-phosphatase motif into a plant homologue improves halotoleranceand drought tolerance of plants [Elektronski vir]Buh Gašparič, Meti ...Soil salinity and drought are among the most serious agricultural and environmental problems of today. Therefore,investigations of plant resistance to abiotic stress have received a lot of attention ... in recent years. In this study, we identifiedthe complete coding sequence of a 39-phosphoadenosine-59-phosphatase protein, ApHal2, from the halotolerant yeastAureobasidium pullulans. Expression of the ApHAL2 gene in a Saccharomyces cerevisiae hal2 mutant complemented themutant auxotrophy for methionine, and rescued the growth of the hal2 mutant in media with high NaCl concentrations. A21-amino-acids-long region of the ApHal2 enzyme was inserted into the Arabidopsis thaliana homologue of Hal2, the SAL1phosphatase. The inserted sequence included the META motif, which has previously been implicated in increased sodiumtolerance of the Hal2 homologue from a related fungal species. Transgenic Arabidopsis plants overexpressing this modifiedSAL1 (mSAL1) showed improved halotolerance and drought tolerance. In a medium with an elevated salt concentration,mSAL1-expressing plants were twice as likely to have roots in a higher length category in comparison with the wild-typeArabidopsis and with plants overexpressing the native SAL1, and had 5% to 10% larger leaf surface area under moderate andsevere salt stress, respectively. Similarly, after moderate drought exposure, the mSAL1-expressing plants showed 14%increased dry weight after revitalisation, with no increase in dry weight of the wild-type plants. With severe drought, plantsoverexpressing native SAL1 had the worst rehydration success, consistent with the recently proposed role of SAL1 in severedrought. This was not observed for plants expressing mSAL1. Therefore, the presence of this fungal META motif sequence isbeneficial under conditions of increased salinity and moderate drought, and shows no drawbacks for plant survival undersevere drought. This demonstrates that adaptations of extremotolerant fungi should be considered as a valuable resourcefor improving stress-tolerance in plant breeding in the future.Source: PloS one [Elektronski vir]. - ISSN 1932-6203 (Vol. 8, iss. 12, Dec. 2013)Type of material - e-articlePublish date - 2013Language - englishCOBISS.SI-ID - 31021785
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Author | Buh Gašparič, Meti ... |
Title | Insertion of a specific fungal 3'-phosphoadenosine-5'-phosphatase motif into a plant homologue improves halotoleranceand drought tolerance of plants [Elektronski vir] |
Publication date | 2013-12-09 |
COBISS.SI-ID | 31021785 |
Publication version in repository | Publisher's version |
Publication licence | Creative Commons Attribution 3.0 Unported |
Embargo | Immediate publication for public |
Project(s) from which the publication was funded
Title | Acronym | Project ID | Funder |
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Funkcionalna analiza proteinov za odpornost proti suši ali žuželkam | J4-2022-2009 |
Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije |
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Molekulski mehanizmi uravnavanja celičnih procesov v povezavi z nekaterimi boleznimi pri človeku | P1-0170-2009 |
Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije |
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Biotehnologija in sistemska biologija rastlin | P4-0165-2009 |
Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije |
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Identifikacija genov iz halotolerantnih gliv za izboljšanje tolerance na sol in sušo pri rastlinah | Z4-5531-2013 |
Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije |
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Database name | Field | Year |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
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Buh Gašparič, Meti | 27502 |
Lenassi, Metka, 1978- | 24288 |
Gostinčar, Cene | 25974 |
Rotter, Ana | 27503 |
Plemenitaš, Ana, 1952- | 06777 |
Gunde-Cimerman, Nina | 05935 |
Gruden, Kristina | 12688 |
Žel, Jana | 03765 |
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