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Ekstraktivi v lesu debel, grč in vej bele jelke ter njihov fungicidni potencial : magistrsko delo = Extractives in wood od stems, knots and branches in silver fir and their fungicidal potential : M. Sc. thesisKeržič, EliAnalizirali smo kemijsko sestavo ekstraktivov v beljavi, jedrovini, grčah in vejah bele jelke (Abies alba Mill.) ter preučili njihove fungistatične, antimikrobne in antioksidativne lastnosti. ... Preiskali smo variabilnost v vsebnosti ekstraktivov v centripetalni smeri, torej od perifernega dela debla proti strženu, ter v longitudinalni smeri. Izolirali smo lesne vzorce debla ter vzorce grč in vej. Jelovino smo ekstrahirali pri povišani temperaturi in tlaku. Kot topili smo uporabili cikloheksan in aceton. Vsebnosti ekstraktivov in celokupnih fenolov smo določili z gravimetrično in s spektrofotometrično metodo. Fenolne ekstraktive smo identificirali s tankoplastno kromatografijo in s tekočinsko kromatografijo visoke ločljivosti. Fungistatične, antimikrobne in antioksidativne lastnosti ekstraktivov smo ocenili z difuznim testom in z metodo lovljenja difenilpikrilhidrazilnega radikala. Ugotovili smo, da največ hidrofilnih ekstraktivov vsebuje les grč in vej, manj jedrovina, najmanj pa beljava. Med identificiranimi spojinami so lignani, fenolne kisline in flavonoidi. V živih grčah in vejah je bilo največ sekoizolaricirezinola, v jedrovini pa matairezinola. Največ fenolnih spojin smo ekstrahirali iz delov grč, ki so vključene v beljavo ter delov vej tik ob deblu. Jedrovina je vsebovala več lignanov v njenih starejših delih. Značilne variabilnosti v vsebnosti ekstraktivov v longitudinalni smeri debla nismo ugotovili. Hidrofilni ekstrakti grč in vej so inhibirali razvoj gliv razkrojevalk lesa ter plesni, vendar pa inhibicija ni bila močna. Ekstrakti grč in vej so izkazali dobre antioksidativne lastnosti. Ugotovili smo, da so ekstrakti grč in vej boljši lovilci prostih radikalov kot ekstrakti jedrovine. Rezultati pričujoče raziskave opisujejo les grč in vej kot najbogatejši vir fenolnih ekstraktivov in lignanov v drevesu bele jelke. Rezultati testov bioloških aktivnosti kažejo na to, da lahko zaščitno funkcijo fenolnih ekstraktivov v lesu bele jelke pojasnimo tudi z njihovimi antioskidativnimi lastnostmi.Type of material - master's thesis ; adult, seriousPublication and manufacture - Ljubljana : [E. Keržič], 2019Language - slovenianCOBISS.SI-ID - 3110025
Author
Keržič, Eli
Other authors
Vek, Viljem |
Oven, Primož
Topics
grče |
bela jelka |
ekstraktivi |
variabilnost |
kromatografija |
naravni antioksidanti |
naravni antioksidanti |
knots |
silver fir |
extractives |
variability |
chromatography |
natural antioxidants |
natural antioxidants
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Library/institution |
City | Acronym | For loan | Other holdings |
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BF, Department of Wood Science and Technology, Lj. | Ljubljana | BFLES |
reading room 1 cop.
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Year | Impact factor | Edition | Category | Classification | ||||
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JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
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DRS, in which the journal is indexed
Database name | Field | Year |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
---|---|
Keržič, Eli | 53624 |
Vek, Viljem | 30758 |
Oven, Primož | 11223 |
Source: Personal bibliographies
and: SICRIS
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