ALL libraries (COBIB.SI union bibliographic/catalogue database)
  • Dominant negative SNARE peptides stabilize the fusion pore in a narrow, release-unproductive state
    Guček, Alenka, 1987- ...
    Key support for vesicle-based release of gliotransmitters comes from studies of transgenic mice with astrocyte-specific expression of a dominant-negative domain of synaptobrevin 2 protein (dnSNARE). ... To determine how this peptide affects exocytosis, we used super-resolution stimulated emission depletion microscopy and structured illumination microscopy to study the anatomy of single vesicles in astrocytes. Smaller vesicles contained amino acid and peptidergic transmitters and larger vesicles contained ATP. Discrete increases in membrane capacitance, indicating single-vesicle fusion, revealed that astrocyte stimulation increases the frequency of predominantly transient fusion events in smaller vesicles, whereas larger vesicles transitioned to full fusion. To determine whether this reflects a lower density of SNARE proteins in larger vesicles, we treated astrocytes with botulinum neurotoxins D and E, which reduced exocytotic events of both vesicle types. dnSNARE peptide stabilized the fusion-pore diameter to narrow, release-unproductive diameters in both vesicle types, regardless of vesicle diameter.
    Source: Cellular and molecular life sciences. - ISSN 1420-682X (Vol. 73, iss. 19, Oct. 2016, str. 3719-3731)
    Type of material - article, component part
    Publish date - 2016
    Language - english
    COBISS.SI-ID - 32590041
    DOI

source: Cellular and molecular life sciences. - ISSN 1420-682X (Vol. 73, iss. 19, Oct. 2016, str. 3719-3731)
loading ...
loading ...
loading ...

Shelf entry