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  • Osmotic effects induced by pore-forming agent nystatin [Elektronski vir] : from lipid vesicles to the cell
    Zemljič Jokhadar, Špela ...
    The responses of Chinese hamster ovary epithelial cells, caused by the pore-forming agent nystatin, were investigated using brightfield and fluorescence microscopy. Different phenomena, i.e., the ... detachment of cells, the formation of blebs, the occurrence of "cell-vesicles" and cell ruptures, were observed. These phenomena were compared to those discovered in giant lipid vesicles. A theoretical model, based on the osmotic effects that occur due to the size-discriminating nystatin transmembrane pores in lipid vesicles, was extended with a term that considers the conservation of the electric charge density in order to describe the cell's behavior. The increase of the cellular volume was predicted and correlated with the observed phenomena.
    Source: PloS one [Elektronski vir]. - ISSN 1932-6203 (Vol. 11, iss. 10, Oct. 2016, str. 1-23)
    Type of material - e-article
    Publish date - 2016
    Language - english
    COBISS.SI-ID - 32962265

    Link(s):

    http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0165098
    DOI

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